Direct association between the yeast Rad51 and Rad54 recombination proteins
Direct association between the yeast Rad51 and Rad54 recombination proteins
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DOI：
10.1074/jbc.271.52.33181
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发表时间：
1996-12-27
影响因子：
4.8
通讯作者：
Kodadek, T
中科院分区：
文献类型：
--
作者：
Jiang, H;Xie, YQ;Kodadek, T
RAD54和RAD51基因参与酿酒酵母中的基因重组和双链断裂修复。Rad51蛋白被认为是大肠杆菌recA基因产物的酵母类似物，并且在体外催化同源单链和双链DNA之间的链交换。RAD54与几种已知的ATP酶具有同源性，并且是SWI2/MOT1家族的成员。我们在此表明，Rad54蛋白在体内和体外都与Rad51蛋白相互作用，并且Rad54蛋白的N末端115个残基对于这种相互作用是必需的。结合先前报道的结果，这些数据暗示Rad54蛋白是多蛋白酵母重组复合物的一部分。
The RAD54 and RAD51 genes are involved in genetic recombination and double-strand break repair in the yeast Saccharomyces cerevisiae. The Rad51 protein is thought to be a yeast analogue of the Escherichia coli recA gene product and catalyzes strand exchange between homologous single- and double-stranded DNAs in vitro, RAD54 exhibits homologies to several known ATPases and is a member of the SWI2/MOT1 family. We show here that the Rad54 protein interacts with the Rad51 protein in vivo and in vitro and that the NH2-terminal 115 residues of the Rad54 protein are necessary for this interaction. Combined with previously reported results, these data imply that the Rad54 protein is part of a multiprotein yeast recombination complex.