Impaired β-Oxidation and Altered Complex Lipid Fatty Acid Partitioning with Advancing CKD
Impaired β-Oxidation and Altered Complex Lipid Fatty Acid Partitioning with Advancing CKD
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DOI：
10.1681/asn.2017030350
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发表时间：
2018-01-01
影响因子：
13.6
通讯作者：
Pennathur, Subramaniam
中科院分区：
文献类型：
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作者：
Afshinnia, Farsad;Rajendiran, Thekkelnaycke M.;Pennathur, Subramaniam
对 CKD（包括 ESRD）中脂质的研究仅限于常规脂质谱的测量。我们的目标是系统地识别 17 种不同的脂质类别，并将其丰度与酰基肉碱（一种 CKD 各个阶段的 β-氧化指标）的变化联系起来。我们从 1235 名成年人的临床表型资源和生物库核心 (CPROBE) 队列中选择了 214 名参与者：36 名患有 1 期或 2 期 CKD，99 名患有 3 期 CKD，61 名患有 4 期 CKD，18 名患有 5 期 CKD。参与者中，110 名男性 (51.4%)、64 名黑人 (29.9%) 和 150 名白人 (70.1%)，平均 (SD) 年龄为 60 (16) 岁。我们使用液相色谱质谱法测量血浆脂质和酰基肉碱。总体而言，我们鉴定了 17 个不同类别的 330 种不同脂质。与早期阶段相比，第 5 阶段 CKD 与更高丰度的饱和 C16-C20 游离脂肪酸 (FFA) 和长链多不饱和复合脂质相关。长链与中链酰基肉碱比率是 β-氧化效率的标志，从 CKD 2 期到 5 期表现出分级下降（P
Studies of lipids in CKD, including ESRD, have been limited to measures of conventional lipid profiles. We aimed to systematically identify 17 different lipid classes and associate the abundance thereof with alterations in acylcarnitines, a metric of beta-oxidation, across stages of CKD. From the Clinical Phenotyping Resource and Biobank Core (CPROBE) cohort of 1235 adults, we selected a panel of 214 participants: 36 with stage 1 or 2 CKD, 99 with stage 3 CKD, 61 with stage 4 CKD, and 18 with stage 5 CKD. Among participants, 110 were men (51.4%), 64 were black (29.9%), and 150 were white (70.1%), and the mean (SD) age was 60 (16) years old. We measured plasma lipids and acylcarnitines using liquid chromatographymass spectrometry. Overall, we identified 330 different lipids across 17 different classes. Compared with earlier stages, stage 5 CKD associated with a higher abundance of saturated C16-C20 free fatty acids (FFAs) and long polyunsaturated complex lipids. Long-chain-to-intermediate-chain acylcarnitine ratio, a marker of efficiency of beta-oxidation, exhibited a graded decrease from stage 2 to 5 CKD (P