Histone protein and DNA synthesis in HeLa cells after thermal shock.
Histone protein and DNA synthesis in HeLa cells after thermal shock.
复制标题
热激后 HeLa 细胞中的组蛋白和 DNA 合成。
DOI：
10.1002/jcp.1041180207
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发表时间：
1984
影响因子：
5.6
通讯作者：
Stone,OL
中科院分区：
文献类型：
--
作者：
Warters,RL;Stone,OL
将悬浮培养的HeLa细胞暴露于45°热冲击导致细胞失活并抑制蛋白质和DNA合成。DNA合成被抑制在一个更敏感的（D0= 7分钟）和一个不太敏感的（D0= 20分钟）相的双相方式。不太敏感的过程被证明是DNA链延长。胸苷进入细胞内池的运输显着不敏感的热休克（D0超过200分钟）。当HeLa细胞在45° C加热15分钟时，前体掺入DNA和蛋白质的抑制率为80%，而前体转运到细胞池中的影响很小。虽然整个细胞和组蛋白（H2a，H2b，H3，和H4）的合成率和DNA链延长细胞加热后6小时恢复，总前体掺入DNA仅为0.4的控制水平。DNA合成速率的长期抑制不能用细胞周期再分配、S期细胞合成DNA的总比例的抑制或DNA链延长速率的抑制来解释。我们得出结论，热冲击导致参与DNA复制的细胞复制子部分的长期抑制。
Exposure of suspension‐cultured HeLa cells to a 45° thermal shock resulted in cell inactivation and inhibition of both protein and DNA synthesis. DNA synthesis was inhibited in a biphasic manner with a more sensitive (D0= 7 min) and a less sensitive (D0= 20 min) phase. The less sensitive process was demonstrated to be DNA chain elongation. Transport of thymidine into intracellular pools was significantly less sensitive to thermal shock (D0in excess of 200 min). When HeLa cells were heated at 45° for 15 min there was an 80% inhibition of incorporation of precursors into both DNA and protein with little effect on precursor transport into cellular pools. While the rate of synthesis of whole cell and histone protein (H2a, H2b, H3, and H4) and DNA chain elongation recovered by 6 h after cell heating, total precursor incorporation into DNA was only 0.4 of control levels. The long‐term depression of the DNA synthetic rate could not be explained by a cell cycle redistribution, a depression in the total fraction of S phase cells synthesizing DNA, or by a depression in the rate of DNA chain elongation. We conclude that thermal shock results in a long‐term depression in the fraction of cell replicons involved in DNA replication.