Reduced Expression of Epidermal Growth Factor Receptor-Related Protein in Hepatocellular Carcinoma: Implications for Cancer Growth
Reduced Expression of Epidermal Growth Factor Receptor-Related Protein in Hepatocellular Carcinoma: Implications for Cancer Growth
复制标题
肝细胞癌中表皮生长因子受体相关蛋白表达减少：对癌症生长的影响
作者：
W. Moon;Kenneth J. Chang;A. Majumdar;A. Tarnawski
背景/目标：表皮生长因子受体（EGFR）的促有丝分裂信号通路在多种癌症中被激活，包括肝细胞癌（HCC）。最近克隆的EGFR相关蛋白（ERRP）被认为是EGFR的负调节因子。ERRP在非恶性肝细胞和HCC中的表达及其与细胞增殖的关系尚未研究。研究方法：用抗ERRP和抗p53抗体对51例HCC和10例正常肝组织的石蜡切片进行免疫组化染色。为了确定ERRP表达与细胞增殖之间的关系，我们在同一HCC标本中进行了ERRP和增殖细胞核抗原（PCNA）的双重染色。结果如下：在正常肝组织中，ERRP在100%的标本中表达，而在来自HCC的非恶性肝组织中，ERRP在50%的标本中表达（p < 0.001）。与此相反，ERRP的表达仅存在于14%的肝癌标本。ERRP在癌细胞中的表达与增殖活性和肿瘤大小呈负相关（分别为p < 0.001，p < 0.05）。p53表达与ERRP表达无明显相关性。结论：人肝癌，这已被证明是与EGFR的激活增加，表现出ERRP表达大幅减少。ERRP表达、肿瘤细胞增殖活性和肿瘤大小之间的负相关关系表明，在某些HCC中，ERRP是HCC生长的负调节因子。
Background/Aims: The mitogenic signaling pathway of epidermal growth factor receptor (EGFR) is activated in a variety of cancers, including hepatocellular carcinoma (HCC). The recently cloned EGFR-related protein (ERRP) has been proposed to be a negative regulator of EGFR. The expression of ERRP in non-malignant liver cells and in HCC, and its relation to cell proliferation, have not been examined. Methods: Paraffin sections of formalin-fixed HCC specimens of 51 HCCs and 10 normal liver specimens were immunostained with anti-ERRP and anti-p53 antibodies. To determine the relationship between ERRP expression and cell proliferation, we performed double staining for ERRP and proliferating cell nuclear antigen (PCNA) in the same HCC specimens. Results: In normal liver, ERRP was expressed in 100% of specimens, while in non-malignant liver tissue from HCC, ERRP expression was present in 50% of the specimens (p < 0.001). In contrast, ERRP expression was present only in 14% of the HCC specimens. The expression of ERRP in cancer cells was inversely correlated with proliferative activity and tumor size (p < 0.001, p < 0.05, respectively). No significant correlation was found between p53 expression and the expression of ERRP. Conclusions: Human HCC, which has been shown to be associated with increased activation of EGFR, exhibits a substantial reduction in ERRP expression. The inverse relationship between ERRP expression, proliferative activity of tumor cells and tumor size suggests that in some HCC, ERRP is a negative regulator of HCC growth.
DOI：
10.1073/pnas.88.22.9979
发表时间：
1991-11-01
影响因子：
11.1
作者：
GINSBERG, D;MECHTA, F;OREN, M
通讯作者：
OREN, M
p53：终极抑癌基因？
DOI：
10.1096/fasebj.6.13.1397838
发表时间：
1992
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
Oren,M
通讯作者：
Oren,M