Replication of Legionella pneumophila in biofilms of water distribution pipes
Replication of Legionella pneumophila in biofilms of water distribution pipes
复制标题
DOI：
10.1016/j.micres.2007.06.001
复制
发表时间：
2009-01-01
影响因子：
6.7
通讯作者：
Ollevier, Frans
中科院分区：
文献类型：
--
作者：
Declerck, Priscilla;Behets, Jonas;Ollevier, Frans
采用由嗜水气单胞菌、大肠杆菌、短黄杆菌和铜绿假单胞菌组成的非军团菌群落，在旋转环形反应器中模拟了与人为水生系统配水管道中相似的生物膜。研究了该菌群与castellanacanthamoeba对嗜肺军团菌复制的影响。尽管存在10(7)个非军团菌，但培养和实时聚合酶链反应（PCR）结果清楚地表明，与生物膜相关的军团菌在castellanii细胞内复制后才增加。生物膜样品的荧光原位杂交（FISH）染色显示，在反应器中加入阿米巴虫48 h后，阿米巴虫群开始裂解，复制的军团菌被释放到散装水中。本研究表明，变形虫类castellanii可在人为水生系统中嗜肺杆菌的增加和传播中发挥关键作用，从而在军团病的发生中发挥关键作用。(C) 2007 Elsevier GmbH版权所有。
Biofilms similar to those present in water distribution pipes of anthropogenic aquatic systems were simulated in a rotating annular reactor using a non-Legionella community consisting of Aeromonas hydrophila, Escherichia coli, Flavobacterium breve and Pseudomonas aeruginosa. The impact of this community and Acanthamoeba castellanii on the replication of Legionella pneumophila was investigated. Despite the presence of 10(7) non-Legionella bacteria, culture and real-time polymerase chain reaction (PCR) results clearly showed that biofilm-associated Legionella bacteria only increased after intracellular replication in A. castellanii. Fluorescent in situ hybridization (FISH) staining of biofilm samples revealed that 48 h after addition of amoebae to the reactor, the amoeba population was lysing and replicated Legionella bacteria were released into the bulk water. This study demonstrated that amoebae like A. castellanii can play a crucial role in the increase and spread of L. pneumophila in anthropogenic aquatic systems and thus in the occurrence of Legionnaires' disease. (C) 2007 Elsevier GmbH. All rights reserved.