Deciphering the metabolic distribution of vanillin in Rhodococcus opacus during lignin valorization
Deciphering the metabolic distribution of vanillin in Rhodococcus opacus during lignin valorization
复制标题
破译木质素增值过程中混色红球菌中香草醛的代谢分布
DOI：
10.1016/j.biortech.2021.126348
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发表时间：
2021
影响因子：
11.4
通讯作者：
Jin Mingjie
中科院分区：
文献类型：
--
作者：
Zhou Huarong;Xu Zhaoxian;Cai Chenggu;Li Jie;Jin Mingjie
香草醛的生物转化是木质素生物增值的重要途径。本研究采用中间体检测、推定基因预测和靶基因验证相结合的方法，对Rhodoccous opacus PD 630中未知的香草醛代谢分布进行了研究。结果表明，在R. opacusPD630.随后，在R. opacusPD 630，其中Pd630_LPD03722产物表现出最大的活性。通过检测香草醛的代谢分布，删除国内的香草醛还原酶基因，引入鞘氨醇菌（Sphingobiumsp.）SYK-6最终，从香草醛的粘康酸产率从最初的10%（mol/mol）增加到97.83%（mol/mol）。此外，本研究还证实了大肠杆菌、枯草芽孢杆菌和谷氨酸棒杆菌中存在香草醛还原酶。
Vanillin bioconversion is important for the biological lignin valorization. In this study, the obscure vanillin metabolic distribution inRhodoccous opacusPD630 was deciphered by combining the strategies of intermediate detection, putative gene prediction, and target gene verification. The results suggest that approximately 10% (mol/mol) of consumed vanillin is converted to vanillic acid for further metabolism, and a large amount is converted to dead-end vanillyl alcohol inR. opacusPD630. Subsequently, five vanillin reductases were identified inR. opacusPD630, among which Pd630_LPD03722 product exhibited the greatest activity. With the detected metabolic distributions of vanillin, the conversion of vanillin to muconic acid was facilitated by deleting domestic vanillin reductase genes and introducing vanillin dehydrogenase fromSphingobiumsp. SYK-6. Ultimately, the muconic acid yield from vanillin increased to 97.83% (mol/mol) from the initial 10% (mol/mol). Moreover, this study demonstrated the existence of vanillin reductases inEscherichia coli,Bacillus subtilis, andCorynebacterium glutamicum.