Evaluation of Nonpathogenic Fusarium oxysporum and Pseudomonas fluorescens for Panama Disease Control
Evaluation of Nonpathogenic Fusarium oxysporum and Pseudomonas fluorescens for Panama Disease Control
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DOI：
10.1094/pdis-06-10-0409
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发表时间：
2011-08-01
期刊：
影响因子：
4.5
通讯作者：
Viljoen, A.
中科院分区：
文献类型：
--
作者：
Belgrove, A.;Steinberg, C.;Viljoen, A.
对采自香蕉健康根部的非致病尖孢镰刀菌内生真菌对香蕉枯萎病(巴拿马病)的防治能力进行了研究。对分离到的菌株进行了形态鉴定，并用种属特异的引物进行了鉴定。在温室内接种香蕉试管苗，证实其致病力。通过基因间隔区聚合酶链式反应限制性片段长度多态性分析，将非致病菌株分为14个单倍型群，并对典型菌株进行生防评价。丘本斯。在温室条件下，10个非致病尖孢霉菌株对香蕉枯萎病有显著的防治效果。对温室香蕉苗保护效果最好的菌株、根际非致病的尖孢霉菌和荧光假单胞菌WCS417进行了田间试验。在田间试验中，无论是非致病的尖孢霉、荧光菌，还是它们的组合，都不能显著抑制枯萎病的发生。许多因素可能导致缺乏田间保护，包括土壤微生物和化学成分以及香蕉根部生防菌存活率下降。对亚热带‘卡文迪什’香蕉枯萎病的病原学和尖孢镰刀菌的作用缺乏了解。小种和香蕉品种对生防菌对香蕉的保护作用有待进一步研究。
Nonpathogenic Fusarium oxysporum endophytes from healthy banana roots were evaluated for their ability to reduce Fusarium wilt of banana (Panama disease). Isolates were identified morphologically and by using species-specific primers. Pathogenicity was confirmed by inoculating banana plantlets in the greenhouse. Nonpathogenic E oxysporum isolates were grouped into 14 haplotype groups by polymerase chain reaction restriction fragment length polymorphism analysis of the intergenic spacer region, and representative isolates evaluated for biocontrol of E oxysporum f. sp. cubense. In the greenhouse, 10 nonpathogenic F oxysporum isolates were able to significantly reduce Fusarium wilt of banana. The isolate that protected banana plantlets best in the greenhouse, a nonpathogenic E oxysporum from the root rhizosphere, and Pseudomonas fluorescens WCS 417 were then field tested. When the putative biological control organisms were tested in the field, neither the nonpathogenic F oxysporum, P fluorescens, nor combinations thereof reduced Fusarium wilt development significantly. A number of factors could contribute to the lack of field protection, including soil microbial and chemical composition and reduced survival of biocontrol organisms in banana roots. A lack of knowledge regarding the etiology of Fusarium wilt of 'Cavendish' banana in the subtropics and the effect of F. oxysporum f. sp. cubense race and banana cultivar in protection of banana by biocontrol organisms should be further investigated.