Spontaneous neuronal discharge patterns in developing organotypic mega-co-cultures of neonatal rat cerebral cortex
Spontaneous neuronal discharge patterns in developing organotypic mega-co-cultures of neonatal rat cerebral cortex
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DOI：
10.1016/j.brainres.2006.05.028
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发表时间：
2006-07-26
期刊：
影响因子：
2.9
通讯作者：
van Pelt, Jaap
中科院分区：
文献类型：
--
作者：
Baker, Robert E.;Corner, Michael A.;van Pelt, Jaap
新生大鼠新皮层的脑片，从前额叶到枕叶区，分别或成对培养，定向在这样一种方式，从每个外植体的脑室表面的轴突投射可以支配其他。功能连接之间以及内的外植体，自发场电位和相关的动作电位发生在变量的突发，并具有不同程度的同步。尖峰序列分析显示，在这些“巨型”共培养物中看到的活动模式密切模仿“轨迹交替”模式，由每分钟重复几次的爆发放电组成，这是慢波睡眠期间未成熟完整大脑皮层的特征。前额叶区域始终不如枕叶区域活跃，但两者在神经元放电模式方面具有质的相似性。另一方面，孤立的巨型文化，尽管他们的大尺寸，只表现出间歇性的短暂爆发，密切类似于枕骨皮质组织碎片和解离细胞培养中观察到的。因此，巨型共培养制剂似乎给出了目前最好的近似体内固有脑放电模式。
Sagittal slices of neonatal rat neocortex, extending from the prefrontal to the occipital area, were cultured separately or in pairs, oriented in such a way that axons projecting from the ventricular surface of each explant could innervate the other one. Functional connections were made between as well as within the explants, and spontaneous field potentials and associated action potentials occurred in variable bursts, and with varying degrees of synchrony. Spike-train analysis revealed that the activity patterns seen in these 'mega' co-cultures closely mimic 'trace alternant' patterns, consisting of trains of burst discharges recurring several times per minute, which are characteristic for the immature intact cerebral cortex during slow-wave sleep. The prefrontal region was consistently less active than the occipital area but the two were qualitatively similar with respect to their patterns of neuronal firing. Isolated mega-cultures, on the other hand, despite their large size, exhibited only intermittent brief bursts that closely resembled those observed both in occipital cortex tissue fragments and in dissociated cell cultures. The mega-co-culture preparation thus appears to give the best currently available approximation to intrinsic cerebral discharge patterns in vivo.