Characterization of CD4+ T cell responses during natural infection with Salmonella typhimurium
Characterization of CD4+ T cell responses during natural infection with Salmonella typhimurium
复制标题
DOI：
10.4049/jimmunol.164.2.986
复制
发表时间：
2000-01-15
影响因子：
4.4
通讯作者：
Jenkins, MK
中科院分区：
文献类型：
--
作者：
McSorley, SJ;Cookson, BT;Jenkins, MK
CD4(+)T细胞在抵抗鼠伤寒沙门氏菌感染中起重要作用。然而，这种T细胞反应的抗原特异性尚不清楚，在这里，我们证明了很大一部分沙门氏菌特异的CD4(+)T细胞对鞭毛细丝蛋白flc有反应，并且这种抗原具有保护幼鼠免受致死性沙门氏菌感染的能力，为了进一步表征这种抗原特异性反应，我们从解决了沙门氏菌减毒株感染的小鼠中克隆了flic特异性的CD4+T细胞。这些克隆被发现对来自一个恒定的flc区域的表位做出反应，使它们能够与许多不同的沙门氏菌血清型表达的鞭毛蛋白发生交叉反应。
CD4(+) T cells are important for resistance to infection with Salmonella typhimurium. However, the Ag specificity of this T cell response is unknown, Here, we demonstrate that a significant fraction of Salmonella-specific CD4(+) T cells respond to the flagellar filament protein, FliC, and that this Ag has the capacity to protect naive mice from lethal Salmonella infection, To characterize this Ag-specific response further, we generated FliC-specific CD4+ T cell clones from mice that had resolved infection with an attenuated strain of Salmonella. These clones were found to respond to an epitope from a constant region of FliC, enabling them to cross-react with flagellar proteins expressed by a number of distinct Salmonella serovars.