Practical guide to the induction of relapsing progressive experimental autoimmune encephalomyelitis in the Biozzi ABH mouse
Practical guide to the induction of relapsing progressive experimental autoimmune encephalomyelitis in the Biozzi ABH mouse
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DOI：
10.1016/j.msard.2011.09.001
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发表时间：
2012-01-01
影响因子：
4
通讯作者：
Baker, David
中科院分区：
文献类型：
--
作者：
Al-Izki, Sarah;Pryce, Gareth;Baker, David
bibiozzi ABH小鼠发展为可重复的、复发缓解型的实验性自身免疫性脑脊髓炎（EAE），随着疾病持续时间的延长成为继发性进行性疾病。观察到的复发是t细胞依赖性的，可以通过免疫耐受诱导来抑制。相反，进行性神经退行性变是T细胞无关的，尽管免疫耐受重新诱导，但仍会继续。在这里，我们提出了在ABH小鼠中诱导EAE的实用指南和用于控制复发的方法，以便探索神经退行性变的自身免疫独立和自身免疫依赖机制。疾病相关性体重变化与血脑屏障功能障碍和临床疾病相关。一种检测神经退行性变的新方法被描述以及新的实验细节，这将有助于在小鼠EAE研究的开展，特别强调对ABH小鼠的研究。(C) 2011 Elsevier B.V.版权所有
Biozzi ABH mice develop a reproducible, relapsing-remitting form of experimental autoimmune encephalomyelitis (EAE) that becomes secondary progressive with disease duration. The relapses observed are T-cell dependent and can be inhibited by immune tolerance induction. In contrast the progressive neurodegeneration is T cell-independent and continues despite the re-induction of immune tolerance. Here we present a practical guide to EAE induction in the ABH mouse and approaches used to control relapses such that both autoimmune-independent and autoimmune-dependent mechanisms of neurodegeneration can be explored. Disease-related weight changes are associated with blood-brain barrier dysfunction and clinical disease. A new method for detecting neurodegeneration is described along with new experimental details that will aid in the undertaking of studies in EAE in mice, with particularly emphasis on ABH mice. (C) 2011 Elsevier B.V. All rights reserved.