Vitamin B6 biosynthesis is essential for the virulence of Xanthomonas oryzae pv. oryzae
Vitamin B6 biosynthesis is essential for the virulence of Xanthomonas oryzae pv. oryzae
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维生素 B6 生物合成对于米黄单胞菌 pv 的毒力至关重要。
DOI：
10.1111/ppa.13708
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发表时间：
2023-02
期刊：
影响因子：
2.7
通讯作者：
Chunxia Li
中科院分区：
文献类型：
--
作者：
Yunyu Hou;Jing Cai;Yunuan Lin;Duo Mi;Jun Tao;Chunxia Li
黄oryzaepv。oryzae（Xoo）是水稻细菌性白叶枯病的致病因子，威胁着世界各地的水稻生产。Xoo对水稻叶片的有效侵染取决于其从植株获取养分和应对寄主防御反应的能力。维生素B6 （VB6）是所有生物生长所必需的，但细菌和植物使用不同的生物合成途径，这意味着维生素B6的生物合成可能是一个有吸引力的抗菌靶点。在细菌中，依赖脱氧木糖5 -磷酸（DXP）和不依赖脱氧木糖5 -磷酸的生物合成途径可以产生VB6。然而，Xoo的VB6生物合成途径尚未被实验确定，其在Xoo毒力中的作用仍不清楚。在此，我们分析了Xoo中VB6的生物合成途径及其在宿主-病原体相互作用中的作用。PdxA和PdxJ是依赖DXP的VB6生物合成的关键成分；我们发现含有这两个假定基因（pdxAandpdxJ）突变的Xoo突变体对VB6完全缺乏营养，这表明Xoo的VB6新生生物合成依赖于DXP。我们还发现这些关键基因（pdxAandpdxJ）的突变显著降低了Xoo在水稻中的毒力。这些结果表明，VB6的新生生物合成对Xoo毒力至关重要。
Xanthomonas oryzaepv.oryzae(Xoo) is the causal agent of rice bacterial blight disease, which threatens rice production around the world. Efficient infection of rice leaves by Xoo depends on its ability to obtain nutrients from the plant and to cope with the host defence responses. Vitamin B6 (VB6) is essential to the growth of all organisms, but bacteria and plants use different biosynthesis pathways, meaning VB6 biosynthesis might be attractive as an antibacterial target. In bacteria, deoxyxylose 5‐phosphate (DXP)‐dependent and deoxyxylose 5‐phosphate‐independent biosynthesis pathways can produce VB6. However, the Xoo VB6 biosynthesis pathway has not been experimentally identified, and its role in Xoo virulence remains unclear. Here, we analysed the VB6 biosynthesis pathway in Xoo and its role in host–pathogen interactions. PdxA and PdxJ are key components for DXP‐dependent VB6 biosynthesis; we found that Xoo mutants containing mutations in these two putative genes (pdxAandpdxJ) were fully auxotrophic for VB6, demonstrating that Xoo VB6 de novo biosynthesis is DXP‐dependent. We also found that mutation of these key genes (pdxAandpdxJ) significantly reduced Xoo virulence in rice. These results suggest that VB6 de novo biosynthesis is essential for Xoo virulence.