In vitro genotoxicity of the West African anti-malarial herbal Cryptolepis sanguinolenta and its major alkaloid cryptolepine
In vitro genotoxicity of the West African anti-malarial herbal Cryptolepis sanguinolenta and its major alkaloid cryptolepine
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DOI：
10.1016/j.tox.2004.11.026
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发表时间：
2005-03-01
期刊：
影响因子：
4.5
通讯作者：
Gooderham, NJ
中科院分区：
文献类型：
--
作者：
Ansah, C;Khan, A;Gooderham, NJ
隐tolepine （CLP）是西非抗疟疾草药Cryptolepis sanguinolenta （Periplocaceae）的主要生物碱，是一种DNA插入剂，在体外对多种哺乳动物细胞表现出强大的毒性。我们假设隐tolepine的DNA嵌入特性可能会引发哺乳动物细胞的遗传损伤。因此，本研究的目的是评估隐tolepine （CLP）和传统的抗疟疾制剂，即根水提取物（CSE）在中国仓鼠成纤维细胞系V79中诱导hprt位点突变和微核（MN）形成的能力。与对照相比，高浓度（50马克杯/毫升）的CSE诱导突变频率明显增加了10倍（平均38个突变克隆/10(6)个存活细胞），但该浓度的CSE毒性很大(
Cryptolepine (CLP), the major alkaloid of the West African anti-malarial herbal Cryptolepis sanguinolenta (Periplocaceae) is a DNA intercalator that exhibits potent toxicity to a variety of mammalian cells in vitro. We have hypothesized that the DNA intercalating properties of cryptolepine could trigger genetic damage in mammalian cells. The objective of the present study was therefore to assess the ability of both cryptolepine (CLP) and the traditional anti-malarial formulation, the aqueous extract from the roots (CSE) to induce mutation at the hprt locus and micronuclei (MN) formation in V79, a Chinese hamster fibroblast cell line commonly used in genetic toxicity studies. CSE at a high concentration (50 mug/ml) induced an apparent significant ten fold increase in mutant frequency compared to vehicle control (mean of 38 versus 4 mutant clones/10(6) surviving cells) but, this concentration of CSE was very toxic (