Increased invasion and tumorigenicity capacity of CD44+/CD24- breast cancer MCF7 cells in vitro and in nude mice.
Increased invasion and tumorigenicity capacity of CD44+/CD24- breast cancer MCF7 cells in vitro and in nude mice.
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DOI：
10.1186/1475-2867-13-62
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发表时间：
2013-06-24
影响因子：
5.8
通讯作者：
Wang T
中科院分区：
文献类型：
--
作者：
Yan W;Chen Y;Yao Y;Zhang H;Wang T
癌症干细胞（CSC）及其行为的鉴定将为未来控制人类癌症提供富有洞察力的信息。本研究在体外和体内研究了 CD44 和 CD24 细胞表面标记作为乳腺癌 CSC 标记。使用带有 CD44 和 CD24 标记的流式细胞术对乳腺癌 MCF7 细胞进行分选，用于扫描电子显微镜 (SEM)、肿瘤细胞侵袭测定和裸鼠异种移植测定。使用 CD44 和 CD24 标记物的流式细胞术分析将 MCF7 细胞分为四个亚群，即 CD44+/CD24-/low、CD44-/CD24+、CD44+/CD24+ 和 CD44-/CD24-。 SEM数据显示CD44+/CD24-/low细胞表面有许多突起。 CD44+/CD24-/low细胞有许多微绒毛和伪足。 CD44+/CD24-/low细胞比其他三个细胞亚群具有更高的迁移和侵袭能力。体内肿瘤形成测定表明，与其他三个亚群相比，CD44+/CD24-细胞具有最高的致瘤能力。 CD44 和 CD24 可能是鉴定乳腺 CSC 的有用标记，因为 CD44+/CD24-/low 细胞具有独特的表面超微结构和最高的致瘤性和侵袭能力。
Identification of cancer stem cells (CSCs) and their behaviors will provide insightful information for the future control of human cancers. This study investigated CD44 and CD24 cell surface markers as breast cancer CSC markers in vitro and in vivo. Flow cytometry with CD44 and CD24 markers was used to sort breast cancer MCF7 cells for scanning electron microscopy (SEM), tumor cell invasion assay, and nude mouse xenograft assay. Flow cytometry assay using CD44 and CD24 markers sorted MCF7 cells into four subsets, i.e., CD44+/CD24-/low, CD44-/CD24+, CD44+/CD24+, and CD44-/CD24-. The SEM data showed that there were many protrusions on the surface of CD44+/CD24-/low cells. CD44+/CD24-/low cells had many microvilli and pseudopodia. The CD44+/CD24-/low cells had a higher migration and invasion abilities than that of the other three subsets of the cells. The in vivo tumor formation assay revealed that CD44+/CD24- cells had the highest tumorigenic capacity compared to the other three subsets. CD44 and CD24 could be useful markers for identification of breast CSCs because CD44+/CD24-/low cells had unique surface ultrastructures and the highest tumorigenicity and invasive abilities.
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DOI：
10.1158/1078-0432.ccr-09-1532
发表时间：
2010-02-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
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作者：
Park SY;Lee HE;Li H;Shipitsin M;Gelman R;Polyak K
通讯作者：
Polyak K
影响因子：
11.2
作者：
Charafe-Jauffret E;Ginestier C;Iovino F;Wicinski J;Cervera N;Finetti P;Hur MH;Diebel ME;Monville F;Dutcher J;Brown M;Viens P;Xerri L;Bertucci F;Stassi G;Dontu G;Birnbaum D;Wicha MS
通讯作者：
Wicha MS
来自原发性人类肿瘤的卵巢癌起始细胞的鉴定和表征
影响因子：
11.2
作者：
Zhang, Shu;Balch, Curt;Nephew, Kenneth P.
通讯作者：
Nephew, Kenneth P.
影响因子：
64.8
作者：
O'Brien, Catherine A.;Pollett, Aaron;Dick, John E.
通讯作者：
Dick, John E.
影响因子：
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作者：
Black, P H
通讯作者：
Black, P H