Protein kinase B (PKB/Akt) is required for the completion of meiosis in mouse oocytes
Protein kinase B (PKB/Akt) is required for the completion of meiosis in mouse oocytes
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DOI：
10.1016/j.ydbio.2007.12.005
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发表时间：
2008-02-01
影响因子：
2.7
通讯作者：
Sato, Eimei
中科院分区：
文献类型：
--
作者：
Hoshino, Yumi;Sato, Eimei
Akt也称为蛋白激酶B，参与许多细胞过程。Akt在两个残基Thr 308和Ser 473处被磷酸化。Thr 308-磷酸化Akt存在于中心粒周围物质中，而Ser 473-磷酸化Akt的定位与中期卵母细胞中微管的定位相似。在注射Thr 308-或Ser 473-磷酸化Akt抗体的卵母细胞中，Spectroms较短且异常。具体而言，Thr 308-和Ser 473-磷酸化Akts单独发挥作用，并且都是组装中期II（MII）纺锤体所必需的。此外，Thr 308-和Ser 473-磷酸化Akts的功能在MII卵母细胞中不同。虽然卵母细胞表现出第二极体（PB 2）发射后注射的Thr-308肽，染色体排列和微管组织异常。相反，注射Ser 473的肽导致PB 2发射失败。这些结果表明，Thr 308-和Ser 473-磷酸化Akts分别参与受精以完成减数分裂，包括不同的作用（即，Ser 473-磷酸化的Akt参与PB 2的发射，而Thr 308-磷酸化的Akt调节微管的组织）。(C)2008年爱思唯尔公司All rights reserved.
Akt, also known as protein kinase B, is implicated in many cellular processes. Akt is phosphorylated at two residues, Thr308 and Ser473. Thr308-phospborylated Akt is present in pericentriolar materials, while localization of Ser473-phosphorylated Akt was similar to that of microtubules in metaphase oocytes. Spindles were shorter and aberrant in oocytes injected with Thr308- or Ser473-phosphorylated Akt antibodies. Specifically, Thr308- and Ser473-phosphorylated Akts function individually and are both necessary to assemble the metaphase II (MII) spindle. Moreover, the functions of Thr308- and Ser473-phosphorylated Akts differ in MII oocytes. Although oocytes exhibited second polar body (PB2) emission after the injection of a peptide for Thr-308, the chromosomal alignment and microtubular organization were aberrant. In contrast, the injection of a peptide for Ser473 caused a failure of PB2 emission. These results suggest that Thr308- and Ser473-phosphorylated Akts are individually involved in fertilization to complete meiosis, including different roles (i.e., Ser473-phosphorylated Akts are involved in PB2 emission, whereas Thr308-phosphorylated Akts regulate the organization of microtubules). (C) 2008 Elsevier Inc. All rights reserved.