Skin test reactivity and Der p-induced interleukin 10 production in patients with asthma or rhinitis infected with Ascaris
Skin test reactivity and Der p-induced interleukin 10 production in patients with asthma or rhinitis infected with Ascaris
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DOI：
10.1016/s1081-1206(10)61070-8
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发表时间：
2006-05-01
影响因子：
5.9
通讯作者：
Cruz, AA
中科院分区：
文献类型：
--
作者：
Ponte, EV;Lima, F;Cruz, AA
背景资料：目的：比较哮喘和鼻炎患者皮肤对吸入性变应原和Der p特异性IL-10产生的反应性。对113名来自地源性蠕虫流行地区的哮喘或鼻炎患者的横断面研究。所有患者均进行粪便检查和吸入性变应原皮肤点刺试验。对53例患者的外周血单个核细胞（PBMC）培养上清进行了Der p特异性IL-10测定。77%的蛔虫感染者和71%的未感染者的皮肤试验结果呈阳性。感染和未感染患者PBMC培养物中Der p特异性IL-10的中位水平相似（分别为7.8和8.4 pg/mL）。缺乏关联仍然寄生虫负荷时，考虑到，当以前的感染证据的患者从uninfected group.Conclusions：在哮喘或鼻炎患者生活在一个城市地区流行的土蠕虫，我们没有发现蛔虫感染和皮肤对空气过敏原的反应之间的关联。此外，PBMC的Der p特异性IL-10产生没有差异。这些阴性结果表明，与血吸虫感染中观察到的情况不同，生活在城市地区的人感染蛔虫不会引起强烈的调节反应。
Background: Helminth infections have been associated with reduced reactivity to aeroallergens, which could be related to interleukin 10 (IL-10) production, as reported in schistosomiasis.Objective: To compare skin responses to aeroallergens with Der p specific IL-10 production in patients with asthma or rhinitis according to Ascaris infection status.Methods: Cross-sectional study of 113 patients with asthma or rhinitis from a region endemic for geohelminths. Stool examinations and skin prick tests to aeroallergens were performed in all the patients. Der p specific IL-10 production was measured in the Supernatant of peripheral blood mononuclear cell (PBMC) cultures of a subsample of 53 patients.Results: Forty-seven patients had Ascaris in their stool samples. Skin test results were positive in 77% of Ascaris-infected patients and in 71% of uninfected individuals. Median levels of Der p specific IL-10 in PBMC cultures of infected and uninfected patients were similar (7.8 and 8.4 pg/mL, respectively). The lack of association remained when parasite load was taken into account and when patients with evidence of previous infection were removed from the uninfected group.Conclusions: In patients with asthma or rhinitis living in an urban area endemic for geohelminths, we found no association between Ascaris infection and skin reactivity to aeroallergens. Furthermore, there was no difference in Der p specific IL-10 production by PBMCs. These negative findings indicate that different from what is observed in Schistosoma infection, Ascaris lumbricoides infection in individuals living in an urban area does not induce strong regulatory responses.