Strategies of professional phagocytes in vivo: unlike macrophages, neutrophils engulf only surface-associated microbes
Strategies of professional phagocytes in vivo: unlike macrophages, neutrophils engulf only surface-associated microbes
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DOI：
10.1242/jcs.082792
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发表时间：
2011-09-15
影响因子：
4
通讯作者：
Herbomel, Philippe
中科院分区：
文献类型：
--
作者：
Colucci-Guyon, Emma;Tinevez, Jean-Yves;Herbomel, Philippe
我们的免疫系统对潜在入侵微生物的早期控制主要取决于其主要的专职吞噬细胞巨噬细胞和中性粒细胞。虽然这两种细胞类型的不同功能已被广泛研究，但在适应性免疫反应开始之前，它们各自对入侵微生物的初始控制的贡献知之甚少。自然半透明的斑马鱼幼体最近成为一种强大的脊椎动物模型，可以可视化体内白细胞和微生物之间的动态相互作用。使用高分辨率实时成像，我们发现巨噬细胞可以有效地吞噬血液或充满液体的体腔中的细菌，而中性粒细胞几乎不能这样做。相比之下，中性粒细胞非常有效地清除表面相关的细菌，但不是液体传播的细菌。因此，未经调理的微生物的物理呈现是中性粒细胞吞噬能力的关键决定因素。中性粒细胞只有在微生物经过它们时才会吞噬它们，这是一种“真空吸尘器”式的行为。中性粒细胞吞噬作用的这种依赖于环境的性质应该与人类传染病特别相关，特别是在接触宿主新微生物的早期阶段。
The early control of potentially invading microbes by our immune system primarily depends on its main professional phagocytes macrophages and neutrophils. Although the different functions of these two cell types have been extensively studied, little is known about their respective contributions to the initial control of invading microorganisms before the onset of adaptive immune responses. The naturally translucent zebrafish larva has recently emerged as a powerful model vertebrate in which to visualise the dynamic interactions between leukocytes and microbes in vivo. Using high-resolution live imaging, we found that whereas macrophages efficiently engulf bacteria from blood or fluid-filled body cavities, neutrophils barely do so. By contrast, neutrophils very efficiently sweep up surface-associated, but not fluid-borne, bacteria. Thus the physical presentation of unopsonised microbes is a crucial determinant of neutrophil phagocytic ability. Neutrophils engulf microbes only as they move over them, in a 'vacuum-cleaner' type of behaviour. This context-dependent nature of phagocytosis by neutrophils should be of particular relevance to human infectious diseases, especially for the early phase of encounter with microbes new to the host.