Effects of grassland afforestation on structure and function of soil bacterial and fungal communities.
Effects of grassland afforestation on structure and function of soil bacterial and fungal communities.
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DOI：
10.1016/j.scitotenv.2019.04.259
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发表时间：
2019-08
期刊：
影响因子：
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通讯作者：
Kaibo Wang;Yongwang Zhang;Zhuangsheng Tang;Z. Shangguan;Fan Chang;Feng'an Jia;Yi-ping Chen;
中科院分区：
文献类型：
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作者：
Kaibo Wang;Yongwang Zhang;Zhuangsheng Tang;Z. Shangguan;Fan Chang;Feng'an Jia;Yi-ping Chen;
草地造林对土壤微生物的结构和功能有很大影响。然而，植树造林如何同时改变土壤真菌和细菌群落及其对生态系统管理的影响的机制知之甚少，特别是在氮有限的生态系统中。利用16S rRNA和ITS rRNA基因高通量测序技术，研究了黄土高原天然油松人工草地造林后土壤性质和土壤微生物的变化。结果表明：草地、林草过渡带和林地土壤细菌多样性差异不显著，而草地土壤真菌多样性显著高于林地和林草过渡带（P<0. 05）。3种土地利用方式下，土壤细菌群落中共有OTU的比例均高于土壤真菌群落。结果表明，草地绿化后，优势细菌由变形菌门向放线菌门转变，优势真菌由子囊菌门向担子菌门转变。TZ的土壤细菌和真菌群落与FL有很大的相似性。此外，在所有检测的土壤性质中，土壤氮（N）对土壤微生物群落的影响更显着。草地造林后土壤氮素的减少导致了土壤微生物群落结构和功能的变化。我们的研究结果表明，同时差异变化的组成和多样性的土壤细菌和真菌群落后，从草原到人工林造林。
Grassland afforestation strongly influences the structure and function of soil microorganisms. Yet the mechanisms of how afforestation could simultaneously alter both the soil fungal and bacterial communities and its implications for ecosystem management are poorly understood, especially in nitrogen-limited ecosystems. Using high-throughput sequencing of 16S rRNA and ITS rRNA genes, the present study investigated the changes in soil properties and soil microorganisms after afforestation of natural grasslands with Chinese pine (Pinus tabuliformis) on the Loess Plateau in China. Results showed that soil bacterial diversity had no significant differences among the grassland (GL), forest–grassland transition zone (TZ), and forestland (FL), while soil fungal diversity in the GL was significantly higher than that in the FL and TZ (P< 0.05). The proportion of shared OTUs in the soil bacterial community was higher than that in the soil fungal community among the three land use types. The dominant bacterial phylum shifted from Proteobacteria to Actinobacteria, while the dominant fungal phylum shifted from Ascomycota to Basidiomycota after the GL conversion to the FL. The functional groups of ECM fungi increased significantly while biotrophic fungi decreased significantly after grassland afforestation. Both the soil bacterial and fungal communities in the TZ showed great similarity with those in the FL. In addition, among all examined soil properties, soil nitrogen (N) showed a more significant effect on the soil microbial communities. The reduction of soil N after grassland afforestation resulted in both the structure and function changes in soil microbial communities. Our results demonstrated simultaneously differential changes in the composition and diversity of both soil bacterial and fungal communities after afforestation from grasslands to planted forests.