Exogenous cytokine modulation or neutralization of interleukin‐10 enhance survival in lipopolysaccharide‐hyporesponsive C3H/HeJ mice with Klebsiella infection
Exogenous cytokine modulation or neutralization of interleukin‐10 enhance survival in lipopolysaccharide‐hyporesponsive C3H/HeJ mice with Klebsiella infection
复制标题
外源细胞因子调节或中和白细胞介素 10 可提高克雷伯氏菌感染的脂多糖低反应性 C3H/HeJ 小鼠的存活率
作者：
Wang;Jeng;Ping
肺炎克雷伯氏菌已从白血病或糖尿病患者的肝脓肿中分离出来。克雷伯氏菌感染在脂多糖（LPS）低反应小鼠中的耐药性尚不清楚。将6-8周龄的雌性C3 H/HeJ和C3 H/HeN小鼠腹膜内（i. p.）注射K.肺炎。结果表明，C3 H/HeJ小鼠对K的易感性[致死剂量50%（LD 50）250集落形成单位]是C3 H/HeN小鼠的24倍。肺炎感染。C3 H/HeJ小鼠，未感染或感染K.肺炎，具有比C3 H/HeN小鼠更高的肝白细胞介素（IL）-10水平和IL-10 mRNA水平。 此前，用IL-1β和肿瘤坏死因子-α（TNF-α）预处理可保护C3 H/HeJ小鼠免受致死性细菌感染。因此，在两种C3 H小鼠品系中检查了感染前1小时腹腔注射IL-1β和TNF-α或抗尿IL-10抗体预处理的影响。 用TNF-α或抗IL-10抗体预处理提高了两种小鼠品系的存活率。TNF-α与IL-1β联合使用，在C3 H/HeJ小鼠中的存活率和细菌清除率高于单独预处理。在感染期间，抗IL-10抗体增加了C3 H/HeJ小鼠中的细菌清除率，并显著降低了肝脏细胞因子mRNA水平。这些结果表明，外源性细胞因子调节或IL-10的中和增强了C3 H/HeJ小鼠对LD 50感染的抵抗力。
Klebsiella pneumoniae has been isolated from liver abscesses in patients with leukaemia or diabetes. The resistance of Klebsiella infection in lipopolysaccharide (LPS)‐hyporesponsive mice is unclear. Female C3H/HeJ and C3H/HeN mice, 6–8 weeks old, were intraperitoneally (i.p.) injected with K. pneumoniae. The results showed that C3H/HeJ mice were 24 times more susceptible [lethal dose 50% (LD50) 250 colony‐forming units] than C3H/HeN mice to K. pneumoniae infection. C3H/HeJ mice, uninfected or infected with K. pneumoniae, had higher liver interleukin (IL)‐10 levels and IL‐10 mRNA levels than C3H/HeN mice. Previously, pretreatment with IL‐1β and tumour necrosis factor‐α (TNF‐α) protected C3H/HeJ mice from lethal bacterial infection. Therefore the effects of pretreatment with IL‐1β and TNF‐α or antimurine IL‐10 antibody i.p. 1 hr before this infection in both strains of C3H mice were examined. Pretreatment with TNF‐α or anti‐IL‐10 antibody enhanced the survival of both strains of mice. TNF‐α, in combination with IL‐1β, enhanced the survival and bacterial clearance better than single pretreatment in C3H/HeJ mice. Anti‐IL‐10 antibody increased bacterial clearance and significantly reduced liver cytokine mRNA levels in C3H/HeJ mice more than it did in the controls during infection. These results indicate that exogenous cytokine modulation or neutralization of IL‐10 enhance the resistance of LD50 infection in C3H/HeJ mice.
C3H/HeJ 和 C3HeB/FeJ 小鼠中单独的遗传缺陷影响革兰氏阴性菌感染易感性的证据。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hagberg,L;Briles,DE;Eden,CS
通讯作者：
Eden,CS
沙门氏菌易感 C3H/HeJ 小鼠的巨噬细胞缺陷和炎症细胞募集功能障碍。
影响因子：
4.3
作者：
Weinstein,DL;Lissner,CR;Swanson,RN;O'Brien,AD
通讯作者：
O'Brien,AD
影响因子：
4.4
作者：
M. Greenberger;R. Strieter;S. Kunkel;J. Danforth;R. Goodman;T. Standiford
通讯作者：
M. Greenberger;R. Strieter;S. Kunkel;J. Danforth;R. Goodman;T. Standiford