Isolation of a cDNA encoding a Kex2-like endoprotease with homology to furin from the nematode Caenorhabditis elegans
Isolation of a cDNA encoding a Kex2-like endoprotease with homology to furin from the nematode Caenorhabditis elegans
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DOI：
10.1089/dna.1997.16.663
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发表时间：
1997-05-01
影响因子：
3.1
通讯作者：
Dickerson, IM
中科院分区：
文献类型：
--
作者：
GomezSaladin, E;Luebke, AE;Dickerson, IM
从秀丽隐杆线虫中分离到一段编码丝氨酸内蛋白酶Kex2家族成员的内蛋白酶的cDNA。根据已知的kex2样内源性蛋白酶活性位点保守区域设计简并寡核苷酸引物，对秀丽隐杆线虫多聚(A)(+)RNA进行逆转录聚合酶链反应（RT-PCR），得到与已知的furin内源性蛋白酶活性位点同源的PCR产物，并作为探针筛选秀丽隐杆线虫cDNA文库。鉴定出一种编码692个氨基酸的前内源性蛋白酶的kex2样内源性蛋白酶（CelfurPC）。CelfurPC的催化结构域的氨基酸序列与已知的ked样内源性蛋白酶同源，与furin/PACE4家族的结构同源性最强。然而，所有的furins和PACE4蛋白都含有一个特征的富含半胱氨酸的结构域，所有的furins都含有一个跨膜结构域，这些都不存在于CelfurPC蛋白中。CelfurPC可能代表了一类新的kex2样内源性蛋白酶。
A cDNA was isolated from the nematode Caenorhabditis elegans that encodes an endoprotease which is a member of the Kex2 family of serine endoproteases. Degenerate oligonucleotide primers were designed based on conserved regions within the active sites of known Kex2-like endoproteases, and were used for reverse transcription-polymerase chain reaction (RT-PCR) of poly(A)(+)RNA isolated from C. elegans, A PCR product was isolated that had homology to the active sites of known furin endoproteases, and was used as a probe to screen a C. elegans cDNA library. A Kex2-like endoprotease (CelfurPC) which encoded a 692-amino-acid preproendoprotease, was identified. The deduced amino acid sequence for the catalytic domain of CelfurPC is homologous to the known Ked-like endoproteases, with strongest structural homology to the furin/PACE4 family, However, all furins and PACE4 proteins contain a characteristic cysteine-rich domain, and all furins contain a transmembrane domain, neither of which is present in the CelfurPC protein. CelfurPC may thus represent a new class of Kex2-like endoprotease.