Progressive DNA demethylation in epigenetic hybrids between parental plants with and without methylation of the transgene promoter
Progressive DNA demethylation in epigenetic hybrids between parental plants with and without methylation of the transgene promoter
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DOI：
10.1007/s00122-021-04004-9
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发表时间：
2022-01
影响因子：
5.4
通讯作者：
W. Matsunaga;T. Inukai;C. Masuta
中科院分区：
文献类型：
--
作者：
W. Matsunaga;T. Inukai;C. Masuta
关键信息：不同DNA甲基化状态的亲本杂交导致f1杂交种的进行性去甲基化。摘要在植物育种研究中，杂种优势是一个非常重要的现象。杂种优势，或称杂种优势，是指亲本特定组合的f1杂种具有优于亲本的性状。此外，DNA甲基化是影响植物基因组基因表达和杂种活力的重要因素。将35S启动子序列导入黄瓜花叶病毒（CMV）载体，用重组病毒特异性接种表达gfp的转基因苯胺碱16c，诱导35S启动子DNA甲基化。对于通过35S启动子甲基化（35S-TGS）建立了GFP转录基因沉默（TGS）的植物，TGS在其后来的自花授粉世代中完全维持。当35S- tgs植株与35S启动子中不含DNA甲基化的16c杂交时，f1杂种出人意料地随着植株的生长逐渐发生DNA去甲基化。我们假设，在由具有极端不同基因甲基化状态的亲本杂交产生的f1杂交种中，随着植物的生长，相关基因的甲基化状态可能越来越多地转向低甲基化。本研究中观察到的这种进行性去甲基化现象可能对植物育种中被DNA甲基化沉默的基因的重新激活具有重要意义。
Key messageCrosses of parents that differ in their DNA methylation states leads to progressive demethylation in the F1hybrids.AbstractIn plant breeding research, hybrid vigor in F1hybrids is known to be a very important phenomenon. Hybrid vigor, or heterosis, refers to the fact that F1hybrids from crosses with a certain combination of parents have traits that are superior to those of the parents. In addition, DNA methylation is an important factor that affects gene expression in plant genomes and contributes to hybrid vigor. We introduced the 35S promoter sequence into the cucumber mosaic virus (CMV)-based vector and inoculated the GFP-expressing transgenicNicotiana benthamianaline 16c with the recombinant virus specifically to induce DNA methylation on the 35S promoter. For plants that had transcriptional gene silencing (TGS) of GFP established by methylation of the 35S promoter (35S-TGS), TGS was fully maintained in their later self-pollinated generations. When the 35S-TGS plants were crossed with 16c, which does not contain DNA methylation in the 35S promoter, the F1hybrids unexpectedly became progressively DNA demethylated as the plants grew. We hypothesis that in F1hybrids that are produced by a cross between parents with extremely different gene methylation states, the methylation state of the genes in question may shift more and more to hypomethylation as the plants grow. This progressive demethylation phenomenon observed in this study may be important in plant breeding to reactivate the genes which were silenced by DNA methylation.