Toxoplasma gondii: difference of invasion into tissue of digestive organs between susceptible and resistant strain and influence of IFN-gamma in mice inoculated with the cysts perorally.
Toxoplasma gondii: difference of invasion into tissue of digestive organs between susceptible and resistant strain and influence of IFN-gamma in mice inoculated with the cysts perorally.
复制标题
弓形虫：敏感株和耐药株侵入消化器官组织的差异及IFN-γ对口服包囊小鼠的影响。
DOI：
10.2307/3285841
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
A. Yano
中科院分区：
文献类型：
--
作者：
M. Kobayashi;F. Aosai;H. Hata;H. Mun;Y. Tagawa;Y. Iwakura;A. Yano
鉴于不同遗传背景的近交系小鼠对弓形虫慢性感染的易感性存在显著差异这一观点，我们比较了经口感染福谷包囊后，抗性（BALB/c）和敏感（C57 BL/ 6）小鼠在感染早期消化器官中原虫的分布。此外，为了确定干扰素γ（IFN-γ）对囊肿对消化道的感染性的影响，使用了纯合IFN-γ敲除小鼠。采用定量竞争聚合酶链反应（QC-PCR）检测T。弓形虫在不同的器官在不同的时间后摄入囊。SAG1、T.在感染后24小时，在野生型C57 BL/6小鼠的小肠和盲肠中以及在IFN-γ敲除的C57 BL/6小鼠的整个消化道中检测到gondii特异性基因。BALB/c小鼠在摄入包囊后24小时，QC-PCR中未观察到可检测的反应。IFN-γ基因的破坏对BALB/c小鼠的抗感染能力影响不大，但对T. C57 BL/6小鼠直肠和外周血中弓形虫的含量。
Because it is widely accepted that there is a significant difference in susceptibility to chronic infection by Toxoplasma gondii among inbred mouse strains with different genetic backgrounds, we compared the distribution of the protozoa in digestive organs at early stages of infection between resistant (BALB/c) and susceptible (C57BL/ 6) mice after peroral infection with Fukaya strain cysts. Furthermore, to determine the influence of interferon gamma (IFN-gamma) on the infectivity of the cysts to the digestive tract, homozygous IFN-gamma knockout mice were utilized. Quantitative competitive polymerase chain reaction (QC-PCR) was employed to assess the distribution of T. gondii in different organs at various times after ingestion of cysts. SAG1, a T. gondii-specific gene, was detected in the small intestine and the caecum in wild-type C57BL/6 mice and in the whole digestive tract in IFN-gamma knockout C57BL/6 at 24 hr after infection. No detectable reaction in QC-PCR was observed in BALB/c mice at 24 hr after ingestion of the cysts. Destruction of the IFN-gamma gene showed less effect on the resistance to infection in BALB/c mice, but remarkable augmentation of infectivity of T. gondii to the rectum and peripheral blood was observed in C57BL/6 mice.