Inhibition of myoblast differentiation by Sfrp1 and Sfrp2
Inhibition of myoblast differentiation by Sfrp1 and Sfrp2
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DOI：
10.1007/s00441-008-0574-z
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发表时间：
2008-05-01
影响因子：
3.6
通讯作者：
Levin, Jonathan
中科院分区：
文献类型：
--
作者：
Descamps, Simon;Arzouk, Hayat;Levin, Jonathan
分泌型卷曲相关蛋白（Sfrps）是Wnt信号转导的细胞外调节因子，在发育和致癌过程中发挥重要作用。已知它们在再生肌肉和成肌细胞培养物中上调，但其功能尚不清楚。在这里，我们表明，除了重组Sfrp1或Sfrp2的C2C12细胞系培养物或卫星细胞的原代培养物的结果在肌管形成的抑制，对细胞周期或细胞凋亡没有显着的影响。尽管在汇合时，处理过的和未处理过的培养物在外观上是相同的，但分析表明，为了获得最大效果，必须在细胞增殖时对其进行处理。此外，在分化过程中从培养基中去除Sfrp恢复了正常的肌管形成。我们的结论是，Sfrp1和Sfrp2的行为，以防止成肌细胞进入终末分化过程。
Secreted Frizzled-related proteins (Sfrps) are extracellular regulators of Wnt signalling and play important roles in developmental and oncogenic processes. They are known to be upregulated in regenerating muscle and in myoblast cultures but their function is unknown. Here, we show that the addition of recombinant Sfrp1 or Sfrp2 to C2C12 cell line cultures or to primary cultures of satellite cells results in the inhibition of myotube formation with no significant effect on the cell cycle or apoptosis. Even though at confluence, treated and untreated cultures are identical in appearance, analyses have shown that, for maximum effect, the cells have to be treated while they are proliferating. Furthermore, removal of Sfrp from the culture medium during differentiation restores normal myotube formation. We conclude that Sfrp1 and Sfrp2 act to prevent myoblasts from entering the terminal differentiation process.