A New Glabrous Gene (csgl3) Identified in Trichome Development in Cucumber (Cucumis sativus L.)
A New Glabrous Gene (csgl3) Identified in Trichome Development in Cucumber (Cucumis sativus L.)
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黄瓜（Cucumis sativus L.）毛状体发育中鉴定出的新无毛基因（csgl3）
DOI：
10.1371/journal.pone.0148422
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发表时间：
2016-02-04
期刊：
影响因子：
3.7
通讯作者：
Gu, Xing-Fang
中科院分区：
文献类型：
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作者：
Cui, Jin-Ying;Miao, Han;Gu, Xing-Fang
在中国，黄瓜果实上的刺或毛状体提高了它们的商业价值。此外，无毛突变体表现出对蚜虫的抗性，因此种植者使用它们可以减少农药残留。先前的研究已经报道了两种含有基因csgl 1和csgl 2的无毛突变体植物。在本研究中，一个新的无毛突变体，NCG 157，被确定为显示与csgl 1和csgl 2的基因互作效应。该突变体茎、叶、卷须、花托和子房无毛，果面无刺或瘤。遗传分析表明，无毛性状受一对隐性基因控制，命名为csgl 3。来自两个自交系9930（来自北方中国的一种表现出毛状体的新鲜市场类型）和NCG 157（具有无毛表面的一种美国加工类型）的杂交的F2群体用于csgl 3基因的遗传作图。采用群体分离分析（BAS）和分子标记相结合的方法，筛选出与csgl 3基因连锁的18个标记，包括2个简单重复序列（SSR）、9个插入缺失（InDel）和7个衍生的切割扩增多态性序列（dCAPs）。所有连锁标记均作为锚位点定位黄瓜第6染色体上的csgl 3基因。csgl 3基因定位在dCAPs标记dCAPs-21和dCAPs-19之间，遗传距离分别为0.05 cM和0.15 cM。该区域的物理距离为19.6kb。三个标记InDel-19、dCAPs-2和dCAPs-11与csgl 3共分离。该区域有两个候选基因，Csa 6 M514860和Csa 6 M514870。实时荧光定量PCR结果显示，Csa 6 M514870在9930组织中的表达量高于NCG 157，这与二者的表型特征相一致。因此推测Csa 6 M514870是黄瓜毛状体发育的候选基因。本研究为黄瓜植株光滑性状的分子标记辅助选择及csgl 3基因的克隆奠定了基础。
Spines or trichomes on the fruit of cucumbers enhance their commercial value in China. In addition, glabrous mutants exhibit resistance to aphids and therefore their use by growers can reduce pesticide residues. Previous studies have reported two glabrous mutant plants containing the genes, csgl1 and csgl2. In the present study, a new glabrous mutant, NCG157, was identified showing a gene interaction effect with csgl1 and csgl2. This mutant showed the glabrous character on stems, leaves, tendrils, receptacles and ovaries, and there were no spines or tumors on the fruit surface. Inheritance analysis showed that a single recessive gene, named csgl3, determined the glabrous trait. An F2 population derived from the cross of two inbred lines 9930 (a fresh market type from Northern China that exhibits trichomes) and NCG157 (an American processing type with glabrous surfaces) was used for genetic mapping of the csgl3 gene. By combining bulked segregant analysis (BAS) with molecular markers, 18 markers, including two simple sequence repeats (SSR), nine insertion deletions (InDel) and seven derived cleaved amplified polymorphism sequences (dCAPs), were identified to link to the csgl3 gene. All of the linked markers were used as anchor loci to locate the csgl3 gene on cucumber chromosome 6. The csgl3 gene was mapped between the dCAPs markers dCAPs-21 and dCAPs-19, at genetic distances of 0.05 cM and 0.15 cM, respectively. The physical distance of this region was 19.6 kb. Three markers, InDel-19, dCAPs-2 and dCAPs-11, co-segregated with csgl3. There were two candidate genes in the region, Csa6M514860 and Csa6M514870. Quantitative real-time PCR showed that the expression of Csa6M514870 was higher in the tissues of 9930 than that of NCG157, and this was consistent with their phenotypic characters. Csa6M514870 is therefore postulated to be the candidate gene for the development of trichomes in cucumber. This study will facilitate marker-assisted selection (MAS) of the smooth plant trait in cucumber breeding and provide for future cloning of csgl3.