Regulation of expression of the chicken ovalbumin gene: interactions between steroid hormones and second messenger systems.
Regulation of expression of the chicken ovalbumin gene: interactions between steroid hormones and second messenger systems.
复制标题
鸡卵清蛋白基因表达的调节：类固醇激素和第二信使系统之间的相互作用。
作者：
E. Skoufos;M. M. Sanders
鸡卵清蛋白基因受到多种激素的调节。它的最大表达不仅需要雌激素和皮质酮的协同作用，还需要胰岛素的允许作用。除了对转录的影响外，雌激素还极大地增强了其信息的稳定性。此外，涉及蛋白激酶的两条信号转导通路也参与了卵清蛋白基因的调控。为了更好地确定第二信使在卵清蛋白基因表达中的作用，研究了蛋白激酶C(PKC)和cAMP依赖的蛋白激酶(PKA)通路对卵清蛋白基因内源水平和卵清蛋白融合基因转录的影响。原代培养的输卵管细胞分别用佛波酯(PKC激活剂)、Forsklin和3-异丁基-1-甲基黄嘌呤(PKA激活剂)、激活剂+雌激素和皮质酮、激活剂+类固醇和胰岛素处理。结果表明，佛波醇12-肉豆蔻酸酯-13-乙酸酯引起卵清蛋白信息的急剧失稳，导致卵清蛋白基因表达水平的降低。相反，PKA系统的激活剂可以替代胰岛素，从而与类固醇协同增加卵清蛋白基因的表达。PKA系统激活剂的作用是在转录水平。因此，在鸡输卵管细胞培养中，PKA和PKC信号转导通路以相反的方式调节类固醇诱导的卵清蛋白基因的表达。
The chicken ovalbumin gene is subject to multihormonal regulation. Maximal expression of it requires not only the synergistic effects of estrogen and corticosterone, but also the permissive effects of insulin. In addition to effects on transcription, the stability of its message is greatly enhanced by estrogen. Furthermore, two signal transduction pathways involving protein kinases have been implicated in the regulation of the ovalbumin gene. To better define the role of second messengers on expression of the ovalbumin gene, the effects of the protein kinase-C (PKC) and the cAMP-dependent protein kinase (PKA) pathways on the endogenous levels of ovalbumin mRNA and the transcription of an ovalbumin fusion gene were investigated. Primary cultures of oviduct cells were treated with phorbol 12-myristilate 13-acetate (an activator of PKC) or with forskolin and 3-isobutyl-1-methylxanthine (an activator of PKA) alone, activators plus estrogen and corticosterone, or activators plus both steroids and insulin. The results indicate that phorbol 12-myristilate 13-acetate causes a dramatic destabilization of ovalbumin message, resulting in a reduction in ovalbumin mRNA levels. In contrast, the activators of the PKA system can substitute for insulin and, thereby, increase expression of the ovalbumin gene synergistically with the steroids. The effect of the activators of the PKA system is at the level of transcription. Thus, in chicken oviduct cell cultures, the PKA and PKC signal transduction pathways act in opposing ways to modulate the steroid-induced expression of the ovalbumin gene.
卵清蛋白基因中的类固醇依赖性调节元件并不起到典型类固醇反应元件的作用。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Schweers,LA;Frank,DE;Weigel,NL;Sanders,MM
通讯作者：
Sanders,MM
卵清蛋白基因的调节：胰岛素、3,5-单磷酸腺苷和雌激素的作用。
影响因子：
4.8
作者：
Evans,MI;McKnight,GS
通讯作者：
McKnight,GS
12-O-十四烷酰佛波醇-13-乙酸酯对 MCF-7 细胞中雌激素受体 mRNA 的转录后去稳定作用。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Saceda,M;Knabbe,C;Dickson,RB;Lippman,ME;Bronzert,D;Lindsey,RK;Gottardis,MM;Martin,MB
通讯作者：
Martin,MB