PROLIFERATING SUBVENTRICULAR ZONE CELLS IN THE ADULT MAMMALIAN FOREBRAIN CAN DIFFERENTIATE INTO NEURONS AND GLIA
PROLIFERATING SUBVENTRICULAR ZONE CELLS IN THE ADULT MAMMALIAN FOREBRAIN CAN DIFFERENTIATE INTO NEURONS AND GLIA
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DOI：
10.1073/pnas.90.5.2074
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发表时间：
1993-03-01
影响因子：
11.1
通讯作者：
ALVAREZBUYLLA, A
中科院分区：
文献类型：
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作者：
LOIS, C;ALVAREZBUYLLA, A
在成年哺乳动物的端脑中，室下区(SVZ)细胞在体内自发增殖。几项研究表明，SVZ细胞在有丝分裂成神经元或胶质细胞后不会分化，而是会死亡。在目前的工作中，我们证明了在成年小鼠脑中用[H-3]胸腺嘧啶核苷标记的SVZ细胞在外植体培养中直接分化为神经元和胶质细胞。体外[H-3]胸腺嘧啶核苷标记显示，从SVZ外植体分化的神经元中，98%来自体内最后一次分裂的前体细胞。这份报告确认SVZ细胞是成年哺乳动物大脑中的神经元前体细胞。
Subventricular zone (SVZ) cells proliferate spontaneously in vivo in the telencephalon of adult mammals. Several studies suggest that SVZ cells do not differentiate after mitosis into neurons or glia but die. In the present work, we show that SVZ cells labeled in the brains of adult mice with [H-3]thymidine differentiate directly into neurons and glia in explant cultures. In vitro labeling with [H-3]thymidine shows that 98% of the neurons that differentiate from the SVZ explants are derived from precursor cells that underwent their last division in vivo. This report identifies the SVZ cells as neuronal precursors in an adult mammalian brain.