Detergent Effects on Membranes at Subsolubilizing Concentrations: Transmembrane Lipid Motion, Bilayer Permeabilization, and Vesicle Lysis/Reassembly Are Independent Phenomena
Detergent Effects on Membranes at Subsolubilizing Concentrations: Transmembrane Lipid Motion, Bilayer Permeabilization, and Vesicle Lysis/Reassembly Are Independent Phenomena
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DOI：
10.1021/la904194a
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发表时间：
2010-05-18
期刊：
影响因子：
3.9
通讯作者：
Goni, Felix M.
中科院分区：
文献类型：
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作者：
Ahyayauch, Hasna;Bennouna, Mohammed;Goni, Felix M.
已知可溶性两亲物或去污剂对膜产生许多结构和动力学效应，甚至在低于引起膜溶解的那些浓度下（即在所谓的去污剂膜相互作用的阶段I中）。对膜的主要亚溶解洗涤剂效应是跨膜脂质运动（触发器）、膜渗透性屏障的破坏（泄漏）和囊泡裂解/重组。为了正确理解去污剂对膜的增溶作用，重要的是要评估在亚增溶表面活性剂浓度下观察到的各种效应是相互独立发生的，还是通过因果关系相互关联的，以便它们可以被解释为增溶整个过程中的必要步骤。为了回答这个问题，我们探讨了上述三种影响（即，翻转、泄漏和裂解/重组），除了在模型（大单层囊泡）和细胞（红细胞）膜中的溶解。已使用了五种结构不同的表面活性剂，即氯丙嗪、丙咪嗪、Triton X-100、十二烷基硫酸钠和脱氧胆酸钠。他们每个人都以独特的方式行事。我们的研究结果表明，脂质触发器，囊泡泄漏，囊泡裂解/重组发生独立的它们之间和相对于双层增溶，使它们不能被认为是必要的部分，一个更高的统一的膜溶解过程中的洗涤剂。
Soluble amphiphiles, or detergents are known to produce a number of structural and dynamic effects on membranes, even at concentrations below those causing membrane solubilization (i.e. in the so-called stage I of detergent membrane interaction). The main subsolubilizing detergent effects on membranes are transmembrane lipid motion (flip-flop), breakdown of the membrane permeability barrier (leakage), and vesicle lysis/reassembly. For a proper understanding of membrane solubilization by detergents, it is important to assess whether the various effects seen at subsolubilizing surfactant concentrations occur independently from each other or are interconnected by cause effect relationships so that they can be interpreted as necessary steps in the overall process of solubilization. To answer this question, we have explored the three above-mentioned effects (i.e., flip-flop, leakage, and lysis/reassembly) apart from solubilization in model (large unilamellar vesicles) and cell (erythrocyte) membranes. Five structurally different surfactants, namely, chlorpromazine, imipramine, Triton X-100, sodium dodecylsulfate, and sodium deoxycholate have been used. Each of them behaves in a unique way. Our results reveal that lipid flip-flop, vesicle leakage, and vesicle lysis/reassembly occur independently between them and with respect to bilayer solubilization so that they cannot be considered to be necessary parts of a higher-order unified process of membrane solubilization by detergents.