Effects of seminal plasma and of its removal and of egg yolk in the diluent on the survival of fresh and frozen-thawed spermatozoa of the Angora goat.
Effects of seminal plasma and of its removal and of egg yolk in the diluent on the survival of fresh and frozen-thawed spermatozoa of the Angora goat.
复制标题
精浆及其去除以及稀释剂中的蛋黄对安哥拉山羊新鲜和冻融精子存活的影响。
DOI：
10.1071/bi9820305
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
S. Salamon
中科院分区：
文献类型：
--
作者：
A. Ritar;S. Salamon
用Tris-glucose培养基稀释两倍后，雄鹿精浆比公羊精浆的环境更差，以维持两种动物新鲜采集的精子的生存能力。稀释雄鹿精浆中的卵黄（9% v/v）在37℃下孵育2 h后引起培养基凝固并导致精子死亡。雄鹿精浆离心（洗涤）去除有利于精子在冻融后的存活，但效果取决于洗涤强度。当精液被稀释6倍或11倍时，两次洗涤比一次洗涤更有效。然而，后一种方法在稀释21倍后的效率与双重洗涤11倍延长的效率相似。在含1.5 ~ 12% （v/v）蛋黄的重悬冷冻培养基中，洗涤后的精子存活率高于不含蛋黄的精子。卵黄浓度高于1.5% （v/v）会抑制未洗涤精子的解冻后存活。
After twofold dilution with Tris-glucose medium, buck seminal plasma was a poorer milieu than ram seminal plasma for the maintenance of viability of freshly collected spermatozoa of both species. Egg yolk (9% v/v) in the diluted buck seminal plasma caused coagulation of the medium coupled with death of spermatozoa after 2 h incubation at 37 degrees C. Removal of seminal plasma by centrifugation (washing) of buck semen was beneficial for the survival of spermatozoa after freeze-thawing, but the effect depended on the intensity of washing. When the semen was diluted 6- or 11-fold, double washing was more effective than single washing. However, the efficiency of the latter method, after 21-fold dilution, was similar to that of double washing at 11-fold extension. Survival of washed spermatozoa was better when the resuspending-freezing medium contained 1.5-12% (v/v) egg yolk than no egg yolk. Egg yolk concentration higher than 1.5% (v/v) depressed the post-thawing survival of non-washed spermatozoa.