Nanodiagnostics: fast colorimetric method for single nucleotide polymorphism/mutation detection
Nanodiagnostics: fast colorimetric method for single nucleotide polymorphism/mutation detection
复制标题
DOI：
10.1049/iet-nbt:20070001
复制
发表时间：
2007-08-01
影响因子：
2.3
通讯作者：
Baptista, P.
中科院分区：
文献类型：
--
作者：
Doria, G.;Franco, R.;Baptista, P.
纳米科学的进步正在许多研究领域产生重大影响。新的纳米技术的影响在生物诊断学中特别大，其中已经引入了许多基于纳米颗粒的测定用于生物分子检测。迄今为止，纳米粒子的应用主要集中在DNA功能化的金纳米粒子作为目标特异性探针。这些基于金纳米颗粒的系统可用于检测DNA（病原体检测、突变和/或单核苷酸多态性的表征）或RNA（无需事先逆转录和扩增）的特定序列。本文报道了一种快速、廉价的基于纳米颗粒的DNA样品单碱基错配检测（单核苷酸多态性/突变）方法。用与DNA靶互补的巯基修饰的寡核苷酸衍生的金纳米颗粒-Au-纳米探针-用于区分完全互补的错配序列，具有单碱基错配。作者已经成功地应用这种策略来检测β-珠蛋白基因内的常见突变。
Advances in nanosciences are having a significant impact in many areas of research. The impact of new nanotechnologies has been particularly large in biodiagnostics, where a number of nanoparticle-based assays have been introduced for biomolecules detection. To date, applications of nanoparticles have largely focused on DNA-functionalised gold nanoparticles used as the target-specific probes. These gold nanoparticle-based systems can be used for the detection of specific sequences of DNA (pathogen detection, characterisation of mutation and/or single nucleotide polymorphisms) or RNA (without prior retro-transcription and amplification). Here a rapid and inexpensive nanoparticle-based method for single-base mismatch detection (single nucleotide polymorphism/mutation) in DNA samples is reported. Gold nanoparticles derivatised with thiol modified oligonucleotides complementary to DNA targets - Au-nanoprobes - are used to distinguish fully complementary from mismatched sequences, with a single-base mismatch. The authors have successfully applied this strategy to detect common mutations within the beta-globin gene.