Structural characterization and expression analysis of the Neurospora conidiation gene con-6.
Structural characterization and expression analysis of the Neurospora conidiation gene con-6.
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脉孢菌分生孢子基因 con-6 的结构特征和表达分析。
DOI：
10.1006/dbio.1993.1303
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发表时间：
1993
影响因子：
2.7
通讯作者：
Yanofsky,C
中科院分区：
文献类型：
--
作者：
White,BT;Yanofsky,C
粗糙脉孢菌（Neurosporacrassa）的基因con-6在无性孢子（分生孢子）形成过程中表达，但在菌丝体中不表达，在分生孢子形成过程中出现，分生孢子形成后期和成熟分生孢子中表达量较高。CON 6多肽和CON 6-βGal融合蛋白仅在游离分生孢子中以高水平存在。孢子萌发后不久，CON-6 mRNA消失，CON-6多肽被降解。CON 6是一种含有重复序列的小的亲水性多肽;它与任何已知的蛋白质都不同源，但具有类似于玉米胚胎发生后期丰富蛋白质的特征。通过重复点突变过程灭活con-6对分生孢子的形成或萌发没有明显的影响。con-6和其他同源基因的上游序列比较发现了一个共同的潜在调控序列，命名为CRS-B。DNA迁移率变化分析与细胞提取物确定了一个因素，结合到合成的DNA片段含有这个序列。这种结合因子存在于菌丝体中，但不存在于分生孢子培养物中。用独立整合的con-6′-′ lacZ翻译融合体进行的实验揭示了携带相同融合体构建体的转化体之间表达的显著差异。这种变异性可能是由于其他人指出的转化体DNA的差异甲基化造成的。
The genecon-6ofNeurospora crassais expressed during the formation of asexual spores (conidia), but it is not expressed in mycelium.con-6mRNA appears upon induction of conidiation and reaches high levels at the late stages of conidiation, and in mature conidia. The CON6 polypeptide and a CON6-βGal fusion protein were present at high levels only in free conidia. Shortly after spore germinationcon-6mRNA disappears and the CON6 polypeptide is degraded. CON6 is a small, hydrophilic polypeptide containing a repeat sequence; it not homologous to any known protein but has features resembling the late embryogenesis abundant proteins of maize. Inactivation ofcon-6by the repeat-induced point mutation process had no demonstrable effect on formation or germination of conidia. Upstream sequence comparisons forcon-6and othercongenes identified a common potential regulatory sequence, designated CRS-B. DNA mobility shift analyses with cell extracts identified a factor that bound to synthetic DNA fragments containing this sequence. This binding factor was present in mycelium but not in conidiating cultures. Experiments with independent integratedcon-6′-'lacZtranslational fusions revealed substantial variability of expression among transformants carrying identical fusion constructs. This variability may be due to the differential methylation of transformant DNA noted by others.