Anthocyanin Biosynthesis and Methylation of the MdMYB10 Promoter Are Associated with the Red Blushed-Skin Mutant in the Red Striped-Skin "Changfu 2" Apple
Anthocyanin Biosynthesis and Methylation of the MdMYB10 Promoter Are Associated with the Red Blushed-Skin Mutant in the Red Striped-Skin "Changfu 2" Apple
复制标题
DOI：
10.1021/acs.jafc.9b07098
复制
发表时间：
2020-04-15
影响因子：
6.1
通讯作者：
Yuan, Yongbing
中科院分区：
文献类型：
--
作者：
Jia, Dongjie;Li, Zhenghuan;Yuan, Yongbing
苹果皮的颜色，特别是花青素的颜色，是决定市场接受度的关键因素。不同肤色苹果（即，有条纹的和脸红的）进行分析。在红蓝皮突变体（CF-B1和CF-B2）和红条皮亲本（CF-S1和CF-S2）中，共检测到14种花色苷和5种原花青素，其中13种差异显著。红皮苹果花青苷积累量显著高于亲本。花青苷合成基因和调控因子（MdMYB 10、MdbHLH 3和MdWD 40）的转录水平与果实脱标签后4、6和8 d的着色期不同的果皮颜色模式相关。MdMYB 10基因启动子区-1203 ~-779 bp、-1667 ~-1180 bp和-2295 ~-1929 bp的甲基化水平与不同的果皮颜色模式相关，红纹皮苹果甲基化程度较高。这些结果加深了我们对不同果皮类型苹果花青苷积累及其分子机制的理解，从而为苹果育种提供了有价值的信息。
The color of apple skin, particularly anthocyanin-based coloration, is a key factor determining market acceptance. The mechanisms of anthocyanin accumulation in apples with different skin color patterns (i.e., striped and blushed) were analyzed. In total, 14 anthocyanins and 5 procyanidins were simultaneously assayed in red blushed-skin mutants (CF-B1 and CF-B2) and red striped-skin parents (CF-S1 and CF-S2), and 13 significant differences were revealed. Anthocyanin accumulation was significantly higher in the red blushed-skin apples than it was in the parents. The transcript levels of anthocyanin biosynthesis genes and regulatory factors (MdMYB10, MdbHLH3, and MdWD40) were associated with different skin color patterns during the coloring period at 4, 6, and 8 days after the fruits were debagged. The methylation levels of the MdMYB10 promoter regions -1203 to -779 bp, -1667 to -1180 bp, and -2295 to -1929 bp were associated with different skin color patterns, and there was more methylation in red striped-skin apples. These results improve our understanding of anthocyanin accumulation and its underlying molecular mechanism in apples with different skin color patterns, thereby providing valuable information for apple breeding.