Melatonin protects against ionizing radiation‐induced oxidative damage in corpus cavernosum and urinary bladder in rats
Melatonin protects against ionizing radiation‐induced oxidative damage in corpus cavernosum and urinary bladder in rats
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DOI：
10.1111/j.1600-079x.2004.00161.x
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发表时间：
2004-11
影响因子：
10.3
通讯作者：
G. Şener;B. Atasoy;Y. Ersoy;S. Arbak;M. Şengöz;B. Yeğen
中科院分区：
文献类型：
--
作者：
G. Şener;B. Atasoy;Y. Ersoy;S. Arbak;M. Şengöz;B. Yeğen
摘要：本研究旨在探讨药物剂量褪黑素对全身照射(IR)大鼠海绵体和膀胱组织的潜在辐射防护作用。32只雄性SD大鼠接受直线加速器照射，在距皮肤100厘米的焦点处产生6 mV光子。在氯胺酮麻醉下，每只大鼠接受800cGy一次全身照射。分别在IR前和IR后立即给予生理盐水或褪黑素(20和10 mg/kg，i.p)治疗。照射后12小时断头。另一组大鼠在IR后被跟踪72小时，每天重复注射褪黑素一次。对海绵体和膀胱组织中脂质过氧化指标丙二醛(MDA)和关键抗氧化剂谷胱甘肽(GSH)的水平进行了评估。组织也进行了显微镜检查。结果表明，IR后12小时和72小时，两种组织中丙二醛水平均升高(P<；0.001)，而谷胱甘肽水平均降低(P<；0.01)。另一方面，褪黑素显著逆转了这些变化(P<；0.05-0.01)，并伴随着组织学外观的改善。我们的结果表明，全身照射会导致泌尿生殖系统组织的氧化损伤。由于褪黑素的应用逆转了氧化器官损伤，根据生化和组织病理学的结果，提示癌症患者辅以褪黑素辅助治疗可能对放射治疗的成功有一定的好处。
Abstract: The objective of this study was to examine the potential radioprotective properties of pharmacological doses of melatonin on corpus cavernosum and bladder tissues of whole‐body irradiated (IR) rats. A total of 32 male Sprague–Dawley rats were exposed to irradiation performed with a LINAC which produced 6 MV photons at a focus 100 cm distant from the skin. Under ketamine anesthesia, each rat received a single whole‐body dose of 800 cGy. Immediately before and after IR, rats were treated with either saline or melatonin (20 and 10 mg/kg, i.p.) and decapitated at 12 hr after exposure to irradiation. Another group of rats was followed for 72 hr after IR, where melatonin injections were repeated once daily. Tissue levels of malondialdehyde (MDA), an index of lipid peroxidation, and glutathione (GSH), a key antioxidant, were estimated in corpus cavernosum and urinary bladder. Tissues were also examined microscopically. The results demonstrate that both 12 and 72 hr following IR, tissue levels of MDA were elevated (P < 0.001), while GSH levels were reduced (P < 0.01) in both tissues. On the other hand, melatonin reversed these changes significantly (P < 0.05–0.01), concomitant with the improvement in histological appearances. Our results show that whole‐body irradiation causes oxidative damage in the tissues of the genitourinary system. As melatonin administration reversed oxidative organ injury, as assessed by biochemical and histopathological findings, it is suggested that supplementing cancer patients with adjuvant therapy of melatonin may have some benefit for successful radiotherapy.