Peptidoglycan inhibits progesterone and androstenedione production in bovine ovarian theca cells
Peptidoglycan inhibits progesterone and androstenedione production in bovine ovarian theca cells
复制标题
肽聚糖抑制牛卵巢卵泡膜细胞中孕酮和雄烯二酮的产生
DOI：
10.1016/j.tiv.2014.04.005
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发表时间：
2014
影响因子：
3.2
通讯作者：
Shimizu T
中科院分区：
文献类型：
--
作者：
Magata F;Horiuchi M;Miyamoto A;Shimizu T
产后奶牛子宫细菌感染扰乱子宫和卵巢功能。革兰氏阳性菌产生的肽聚糖（PGN）已被证明会破坏母羊的卵巢功能。本研究的目的是确定PGN对卵泡发育不同阶段牛卵泡膜细胞类固醇产生的影响。从选择前和选择后的卵泡（<8.5 mm and >直径分别为8.5 mm）中分离牛卵泡膜细胞，体外培养并用PGN攻击。通过测量培养48小时或96小时后培养基中的孕酮（P4）和雄烯二酮（A4）浓度来评价类固醇产生。牛卵泡膜细胞表达PGN受体，包括Toll样受体2和核苷酸结合寡聚化结构域1和2。用PGN（1、10或50 μg/ml）处理导致选择前和选择后卵泡中卵泡膜细胞产生P4和A4的减少。PGN处理降低了类固醇生成酶的mRNA表达。同时用PGN和脂多糖（0.1、1、10 μg/ml）处理选择后卵泡的卵泡膜细胞，进一步抑制A4的产生。这些研究结果表明，细菌毒素可能局部作用于卵巢类固醇生成细胞，并损害产后奶牛卵泡发育。
Uterine bacterial infection perturbs uterine and ovarian functions in postpartum dairy cows. Peptidoglycan (PGN) produced by gram-positive bacteria has been shown to disrupt the ovarian function in ewes. The aim of this study was to determine the effect of PGN on steroid production in bovine theca cells at different stages of follicular development. Bovine theca cells isolated from pre- and post-selection ovarian follicles (<8.5 mm and >8.5 mm in diameter, respectively) were culturedin vitroand challenged with PGN. Steroid production was evaluated by measuring progesterone (P4) and androstenedione (A4) concentration in culture media after 48 h or 96 h of culture. Bovine theca cells expressed PGN receptors including Toll-like receptor 2 and nucleotide-binding oligomerization domain 1 and 2. Treatment with PGN (1, 10, or 50 μg/ml) led to a decrease in P4 and A4 production by theca cells in both pre- and post-selection follicles. The mRNA expression of steroidogenic enzymes were decreased by PGN treatment. Moreover, A4 production was further suppressed when theca cells of post-selection follicles were simultaneously treated by PGN and lipopolysaccharide (0.1, 1, or 10 μg/ml). These findings indicate that bacterial toxins may act locally on ovarian steroidogenic cells and compromise follicular development in postpartum dairy cows.