A Comparison of DNA Extraction Using AutoMate Express™ and EZ1 Advanced XL from Liquid Blood, Bloodstains, and Semen Stains
A Comparison of DNA Extraction Using AutoMate Express™ and EZ1 Advanced XL from Liquid Blood, Bloodstains, and Semen Stains
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使用 AutoMate Express™ 和 EZ1 Advanced XL 从液体血液、血迹和精液中提取 DNA 的比较
DOI：
10.1111/1556-4029.12174
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发表时间：
2013
影响因子：
1.6
通讯作者：
K. Sekiguchi
中科院分区：
文献类型：
--
作者：
Koji Fujii;Shota Inokuchi;Tetsushi Kitayama;Hiroaki Nakahara;N. Mizuno;K. Sekiguchi
在这项研究中，使用AutoMate Express™和EZ1 Advanced XL从液态血液、新鲜和陈旧血痕以及新鲜和陈旧精斑中提取DNA。通过实时聚合酶链式反应（PCR）利用D17Z1基因座对提取的DNA进行定量。使用AmpFℓSTR® Identifiler试剂盒进行短串联重复序列（STR）分型。AutoMate Express™从新鲜血痕和新鲜精斑中获得的DNA产量较高，从陈旧血痕和陈旧精斑中获得的产量与EZ1 Advanced XL几乎相同，但从液态血液中获得的产量比EZ1 Advanced XL略低。添加二硫苏糖醇或使用PrepFiler™裂解缓冲液可改善EZ1 Advanced XL对新鲜血痕的提取结果，但对液态血液和陈旧血痕无效。我们的研究结果表明，PrepFiler™裂解缓冲液是AutoMate Express™对新鲜血痕能获得较高DNA产量的主要原因。
In this study, DNA was extracted using an AutoMate Express™ and an EZ1 Advanced XL from liquid blood, fresh and aged bloodstains, and fresh and aged semen stains. Extracted DNA was quantified by real‐time PCR using the D17Z1 locus. Short tandem repeat typing was performed using an AmpFℓSTR® Identifiler kit. The yields of DNA obtained by the AutoMate Express™ were higher from fresh bloodstains and fresh semen stains, almost the same from aged bloodstains and aged semen stains, but slightly lower from liquid blood compared with those obtained by the EZ1 Advanced XL. The addition of dithiothreitol or the use of PrepFiler™ lysis buffer improved the EZ1 Advanced XL results from fresh bloodstains, but not for liquid blood and aged bloodstains. Our results demonstrated that the PrepFiler™ lysis buffer is the main contributor to the higher DNA yields of the AutoMate Express™ for fresh bloodstains.