Ontogeny of enkephalin-like immunoreactivity in the rat hippocampus.
Ontogeny of enkephalin-like immunoreactivity in the rat hippocampus.
复制标题
大鼠海马脑啡肽样免疫反应性的个体发育。
DOI：
10.1016/0306-4522(84)90029-0
复制
发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Karten,HJ
中科院分区：
文献类型：
--
作者：
Gall,C;Brecha,N;Chang,KJ;Karten,HJ
用免疫细胞化学技术对大鼠海马结构内亮氨酸5-脑啡肽样免疫反应性的生后发育进行了分析。从出生到出生后第三天，没有内在的海马元件表现出免疫反应性，虽然标记的轴突被发现在海马伞内，在肺泡内，并在附近的角束。生后第4天，在海马CA3区辐射层可见少量免疫反应阳性神经元，生后第8天，在门、上级的背侧层和起源以及下托内可见少量免疫反应阳性神经元。有一个显着的增加，在出生后的第8和10天在所有领域之间的免疫反应perikarya的发病率，以及标记的神经元的出现在CA1层的齿状回的齿状回和颗粒层。两天后，第一次出现的免疫反应性胞体，强烈的免疫反应性神经元，标记更广泛地比以往任何时候都看到在成人中，遇到在每个子区域的海马。的时空顺序出现的perikaryal免疫反应和短暂的外观强烈的免疫反应神经元如下的神经发生，与免疫反应发展后12 - 14天的最后一个细胞分裂的高峰期为一个给定的海马regions.The发病率的免疫反应perikarya在齿状回定量在大鼠幼崽出生后8至19天。标记神经元的出现遵循时空梯度，这也是该区域神经发生的描述。免疫反应perikarya出现在上锥体层颗粒之前，他们出现在锥体下区和颞极的齿状早于中背腹侧dentate.The侧穿通路径和苔藓纤维轴突，表现出脑啡肽样免疫反应在成年海马结构，不同的年龄免疫反应第一次出现在他们的相对成熟度。免疫反应性出现早在出生后第4天的横向perforant路径，年龄在这些轴突刚刚成长到他们的目标领域，而它是没有发现苔藓纤维内，直到出生后第10天，年龄在苔藓纤维扣制作是先进的和生理能力的苔藓纤维突触与regio下锥体细胞已经建立。后一个观察结果表明，脑啡肽是不必要的苔藓纤维突触的突触传递。
The postnatal development of leucine5-enkephalin-like immunoreactivity within the hippocampal formation of the rat has been analyzed using immunocytochemical techniques. From the day of birth to postnatal day three, no intrinsic hippocampal elements exhibit immunoreactivity although labeled axons are found within the fimbria, within the alveus, and in the vicinity of the angular bundle. On postnatal day 4, a few immunoreactive hippocampal neurons can be seen in stratum radiatum of the region CA3 and by postnatal day 8, within the hilus, strata pyramidale and oriens of regio superior, and the subiculum. There is a dramatic increase in the incidence of immunoreactive perikarya between postnatal days 8 and 10 in all fields as well as the appearance of labeled neurons in CA1 stratum pyramidale and stratum granulosum of the dentate gyrus. Two days after the first appearance of immunoreactive perikarya, intensely immunoreactive neurons, labeled much more extensively than is ever seen in the adult, are encountered in each subfield of the hippocampus. The spatio-temporal order in both the emergence of perikaryal immunoreactivity and the transient appearance of intensely immunoreactive neurons follows that of neurogenesis, with immunoreactivity developing 12–14 days after the peak period of last cell division for a given hippocampal region.The incidence of immunoreactive perikarya in the dentate gyrus was quantified in rat pups ranging from postnatal days 8 to 19. The appearance of labeled neurons followed the spatio-temporal gradients that have been described for neurogenesis in this region as well. Immunoreactive perikarya emerged in the suprapyramidal stratum granulosum prior to their emergence in the infrapyramidal zone and in the temporal pole of the dentate earlier than in the mid-dorsoventral dentate.The lateral perforant path and mossy fiber axons, seen to exhibit enkephalin-like immunoreactivity in the adult hippocampal formation, differ in their relative maturity at the age immunoreactivity first appears. Immunoreactivity appears as early as postnatal day 4 in the lateral perforant path, an age at which these axons are just growing into their target field while it is not found within the mossy fibers until after postnatal day 10, an age at which mossy fiber bouton elaboration is well advanced and physiologically competent mossy fiber synapses with the regio inferior pyramidal cells have been established. The latter observation indicates that enkephalin is not necessary for synaptic transmission at the mossy fiber synapse.