Culturing carotid body cells of adult cats.
Culturing carotid body cells of adult cats.
复制标题
培养成年猫的颈动脉体细胞。
DOI:
10.1007/978-1-4615-2572-1_19
复制
发表时间:
1994
影响因子:
--
通讯作者:
Guilarte,TR
中科院分区:
文献类型:
--
作者:
Shirahata,M;Schofield,B;Chin,BY;Guilarte,TR
Recently patch clamp techniques or optical fluorometric techniques have been applied to freshly dissociated or cultured carotid body glomus cells to understand chemosensory mechanisms of the carotid body. Results were not consistent. The inconsistency of the available data may result from various reasons. Firstly, the studied cells might have been heterogeneous due to the difficulty of differentiating between dissociated glomus cells and sheath cells. Secondly, culture conditions may alter characteristics of the glomus cells. Thirdly, differences in the age and species used to generate the available data make the interpretation more difficult. The purpose of this study is to standardise our culture techniques to overcome these difficulties.