Application of Scan-less Two-Dimensional Confocal Microscopy Based on a Combination of Confocal Slit With Wavelength/Space Conversion
Application of Scan-less Two-Dimensional Confocal Microscopy Based on a Combination of Confocal Slit With Wavelength/Space Conversion
复制标题
基于共焦狭缝与波长/空间转换相结合的无扫描二维共焦显微镜的应用
DOI：
10.1109/jstqe.2018.2869615
复制
发表时间：
2019
影响因子：
4.9
通讯作者：
Yasui Takeshi
中科院分区：
文献类型：
--
作者：
Hase Eiji;Minamikawa Takeo;Miyamoto Shuji;Mizutani Yasuhiro;Iwata Tetsuo;Yamamoto Hirotsugu;Yasui Takeshi
共焦激光显微镜（CLM）由于其共焦特性，可实现二维或三维的光学切片和深度选择性成像，在非接触表面形貌、生物医学成像等领域得到了广泛的应用。线聚焦CLM与一维光谱编码CLM的结合使我们能够在不需要机械扫描的情况下获得二维共焦图像。所谓的无扫描2-D CLM是一种独特的成像方式;然而，还没有尝试将其应用于实际应用。在本文中，我们构建了一个无扫描的二维CLM，图像采集时间为0.23 ms，横向分辨率为1.2 μm，深度分辨率为2.4 μm，并将其应用于不同的应用，以评估其实用潜力。
Confocal laser microscope (CLM) has been widely used in the fields of non-contact surface topography, biomedical imaging, and other applications, as confocality gives two-dimensional (2-D) optical-sectioning or three-dimensional (3-D) imaging capability with depth selectivity. The combination of line-focused CLM with one-dimensional spectral encoding CLM enables us to obtain the 2-D confocal image without the need for mechanical scanning. The so-called scan-less 2-D CLM is a unique imaging modality; however, there have been no attempts to apply this to practical applications. In this paper, we have constructed a scan-less 2-D CLM with an image acquisition time of 0.23 ms, lateral resolution of 1.2 μm, depth resolution of 2.4 μm, and have applied it to different kinds of applications to evaluate its practical potential.