THE ACTIVITY OF PEPSIN, CHYMOTRYPSIN AND TRYPSIN DURING 24-H PERIODS IN THE SMALL-INTESTINE OF GROWING-PIGS
THE ACTIVITY OF PEPSIN, CHYMOTRYPSIN AND TRYPSIN DURING 24-H PERIODS IN THE SMALL-INTESTINE OF GROWING-PIGS
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DOI：
10.1079/bjn19820097
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发表时间：
1982-01-01
影响因子：
3.6
通讯作者：
LOW, AG
中科院分区：
文献类型：
--
作者：
LOW, AG
取22头猪的消化液，平均活重40公斤，在十二指肠、空肠或回肠安装单套可折返式肠管。给猪饲喂三种大致等氮的日粮；它们的主要成分是：大麦、优质小麦内脏和白鱼粉(日粮BWF)；淀粉、蔗糖、玉米油、纤维素和花生(日粮SSG)或酪蛋白(日粮SSC)。在24 h采集期内每小时测定十二指肠消化液胃酶、胰凝乳酶和胰蛋白酶活性。在24 h采集期内，空肠每小时测定一次胰凝乳酶和胰凝乳酶，回肠每隔6 h测定一次胰凝乳酶和胰蛋白酶。十二指肠消化中胃酶、胰凝乳酶和胰蛋白酶在24小时内的总重量在6.2-7.1g之间，相当于先前发表的十二指肠这一部分内源性蛋白质含量估计值的20%-25%。
Digesta were collected from 22 pigs, of 40 kg mean live wt, and fitted with single re-entrant cannulas in either the duodenum, jejunum or ileum. Three approximately isonitrogenous diets were given to the pigs; their main constituents were: barley, fine wheat offal and white fish meal (diet BWF); starch, sucrose, maize oil, cellulose and either groundnut (diet SSG) or casein (diet SSC). The activities of pepsin, chymotrypsin and trypsin were measured every hour in duodenal digesta during 24 h collection periods. Chymotrypsin and trypsin were also measured every hour in jejunal digesta and every 6 h in ileal digesta, during 24 h collection periods. The total weights of pepsin, chymotrypsin and trypsin in duodenal digesta in 24 h periods were between 6.2-7.1 g. This represents 20-25% of previously published estimates of the amounts of endogenous protein in this part of the gut.