Methylation of miRNA genes in the response to temperature stress in Populus simonii.
Methylation of miRNA genes in the response to temperature stress in Populus simonii.
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小叶杨响应温度胁迫的 miRNA 基因甲基化
DOI：
10.3389/fpls.2015.00921
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发表时间：
2015
影响因子：
5.6
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Ci D;Song Y;Tian M;Zhang D
DNA甲基化和miRNAs对非生物胁迫的转录和转录后应答提供了重要的调控。在这项研究中，我们使用甲基化敏感的扩增多态性，以确定1066个位点的差异甲基化反应的温度胁迫小叶杨。在这些位点中，miRBase的BLAST搜索鉴定了7个miRNA基因。实时荧光定量PCR表达分析表明，这些miRNA基因的甲基化模式可能会影响其表达。在已测序的大叶杨基因组中对这些miRNA基因进行注释，发现分别由五个基因（Ptc-MIR 396 e和g、Ptc-MIR 156 i和j以及Ptc-MIR 390 c）产生的miRNA有三个靶基因（Potri.007G090400、Potri.014G042200和Potri.010G176000）。这些靶基因的产物在脂质代谢中起作用以消耗脂质过氧化物。我们还构建了一个基于DNA甲基化与miRNAs、miRNAs与靶基因、靶基因产物及其影响的代谢因子（包括H2 O2、丙二醛、过氧化氢酶（CAT）和超氧化物歧化酶）之间相互作用的网络。我们的研究结果表明，DNA甲基化可能调节miRNA基因的表达，从而影响其靶基因的表达，可能通过miRNA的基因沉默功能，以维持细胞在非生物胁迫条件下的存活。
DNA methylation and miRNAs provide crucial regulation of the transcriptional and post-transcriptional responses to abiotic stress. In this study, we used methylation-sensitive amplification polymorphisms to identify 1066 sites that were differentially methylated in response to temperature stress in Populus simonii. Among these loci, BLAST searches of miRBase identified seven miRNA genes. Expression analysis by quantitative real-time PCR suggested that the methylation pattern of these miRNA genes probably influences their expression. Annotation of these miRNA genes in the sequenced genome of Populus trichocarpa found three target genes (Potri.007G090400, Potri.014G042200, and Potri.010G176000) for the miRNAs produced from five genes (Ptc-MIR396e and g, Ptc-MIR156i and j, and Ptc-MIR390c) respectively. The products of these target genes function in lipid metabolism to deplete lipid peroxide. We also constructed a network based on the interactions between DNA methylation and miRNAs, miRNAs and target genes, and the products of target genes and the metabolic factors that they affect, including H2O2, malondialdehyde, catalase (CAT), and superoxide dismutase. Our results suggested that DNA methylation probably regulates the expression of miRNA genes, thus affecting expression of their target genes, likely through the gene-silencing function of miRNAs, to maintain cell survival under abiotic stress conditions.