STAGES IN PHAGE R17 INFECTION .6. INJECTION OF A PROTEIN AND RNA INTO HOST-CELL
STAGES IN PHAGE R17 INFECTION .6. INJECTION OF A PROTEIN AND RNA INTO HOST-CELL
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DOI：
10.1016/0042-6822(72)90552-1
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发表时间：
1972-01-01
期刊：
影响因子：
3.7
通讯作者：
OCALLAGH.RJ
中科院分区：
文献类型：
--
作者：
KRAHN, PM;PARANCHYCH, W;OCALLAGH.RJ
噬菌体R17在A蛋白上用~ 3 H-组氨酸标记，在RNA上用~（32）P标记。然后进行研究以确定这两种噬菌体组分在R17感染期间的命运。发现噬菌体颗粒与F菌毛细菌在37°下的相互作用导致A蛋白（MW 39，000）裂解成分子量约为24，000和15，000的两个较小组分，并且这些多肽片段与噬菌体RNA一起沿着转移到细胞中。此外，还观察到噬菌体RNA和A蛋白以大约等摩尔量注入细胞，并且它们的渗透动力学相似。有人认为，噬菌体RNA进入宿主细胞的渗透可能涉及A蛋白-RNA复合物的注射，而不是游离RNA。
Phage R17 was specifically labeled in the A protein with3H-histidine, and in the RNA with32P. Studies were then carried out to determine the fate of these two phage components during R17 infection. It was found that the interaction of phage particles with F piliated bacteria at 37° leads to the cleavage of A protein (MW 39,000) into two smaller components of molecular weights about 24,000 and 15,000, and that these polypeptide fragments are transferred into the cell along with the phage RNA. In addition, it was observed that phage RNA and A protein are injected into the cell in approximately equimolar amounts, and that their kinetics of penetration are similar. It was suggested that the penetration of phage RNA into the host cell may involve the injection of an A protein-RNA complex rather than free RNA.