Precise sequence complementarity between yeast chromosome ends and two classes of just-subtelomeric sequences
Precise sequence complementarity between yeast chromosome ends and two classes of just-subtelomeric sequences
复制标题
DOI：
10.1073/pnas.95.11.5906
复制
发表时间：
1998-05-26
影响因子：
11.1
通讯作者：
Britten, RJ
中科院分区：
文献类型：
--
作者：
Britten, RJ
酿酒酵母染色体末端区域(最后20kb)普遍含有与其他染色体末端区域精确序列相似的区块。左和右末端区域是不同的，因为它们之间的序列相似性是反向互补。直接序列相似性发生在左侧末端区域之间，也发生在右侧末端区域之间，但不存在于任何左端和右端之间。除了极小的例外，块内的关系范围从80%到100%匹配。相似区域是熟悉的和不熟悉的重复序列以及可以被认为是“单拷贝”(或更好的“双拷贝”)序列的复合体。将所有末端区域与所有其他染色体、正向互补和反向互补进行比较，并绘制了768个比较图表。看来，末端区域之间的序列交换或复制已经有了广泛的历史。亚端粒序列分为两类。17个染色体末端以Y‘重复终止，15个末端以800个核苷酸的“X2”重复终止，恰好与端粒酶简单重复相邻。除了1号染色体右端更具分歧性外，刚刚-亚端的重复序列彼此非常相似。
The terminal regions (last 20 kb) of Saccharomyces cerevisiae chromosomes universally contain blocks of precise sequence similarity to other chromosome terminal regions. The left and right terminal regions are distinct in the sense that the sequence similarities between them are reverse complements. Direct sequence similarity occurs between the left terminal regions and also between the right terminal regions, but not between any left ends and right ends. With minor exceptions the relationships range from 80% to 100% match within blocks. The regions of similarity are composites of familiar and unfamiliar repeated sequences as well as what could be considered "single-copy" (or better "two-copy") sequences. All terminal regions were compared with all other chromosomes, forward and reverse complement, and 768 comparisons are diagrammed. It appears there has been an extensive history of sequence exchange or copying between terminal regions. The subtelomeric sequences fall into two classes. Seventeen of the chromosome ends terminate with the Y' repeat, while 15 end with the 800-nt "X2" repeats just adjacent to the telomerase simple repeats. The just-subterminal repeats are very similar to each other except that chromosome 1 right end is more divergent.