Translation termination reinitiation between open reading frame 1 (ORF1) and ORF2 enables capsid expression in a bovine norovirus without the need for production of viral subgenomic RNA
Translation termination reinitiation between open reading frame 1 (ORF1) and ORF2 enables capsid expression in a bovine norovirus without the need for production of viral subgenomic RNA
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DOI：
10.1128/jvi.02362-07
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发表时间：
2008-09-01
影响因子：
5.4
通讯作者：
Clarke, Ian N.
中科院分区：
文献类型：
--
作者：
McCormick, Christopher J.;Salim, Omar;Clarke, Ian N.
关于诺如病毒复制的普遍接受的观点是衣壳表达需要产生亚基因组转录物，衣壳的存在通常用作指示病毒复制发生的替代标记。使用基于聚合酶II的杆状病毒递送系统，我们观察到衣壳表达后，引入全长基因组3诺如病毒基因组到HepG 2细胞中。然而，衣壳表达发生作为一个新的翻译终止/重新启动事件之间的非结构蛋白和衣壳开放阅读框架，一个功能，可能是独特的基因组3诺如病毒。
A generally accepted view of norovirus replication is that capsid expression requires production of a subgenomic transcript, the presence of capsid often being used as a surrogate marker to indicate the occurrence of viral replication. Using a polymerase II-based baculovirus delivery system, we observed capsid expression following introduction of a full-length genogroup 3 norovirus genome into HepG2 cells. However, capsid expression occurred as a result of a novel translation termination/reinitiation event between the nonstructural-protein and capsid open reading frames, a feature that may be unique to genogroup 3 noroviruses.