THE GLYCOSYLPHOSPHATIDYLINOSITOL MEMBRANE ANCHOR OF TRYPANOSOMA-BRUCEI VARIANT SURFACE GLYCOPROTEIN
THE GLYCOSYLPHOSPHATIDYLINOSITOL MEMBRANE ANCHOR OF TRYPANOSOMA-BRUCEI VARIANT SURFACE GLYCOPROTEIN
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DOI：
10.1042/bst0160265
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发表时间：
1988-06-01
影响因子：
3.9
通讯作者：
RADEMACHER, TW
中科院分区：
文献类型：
--
作者：
FERGUSON, MAJ;HOMANS, SW;RADEMACHER, TW
真核生物的细胞表面整合膜糖蛋白可以根据附着于质膜的方式进行分类。主要类别采用一个或多个跨膜疏水肽序列;次要类别通过共价键与磷脂酰肌醇磷脂锚定。后一种锚似乎在原生动物中特别常见。布氏锥虫的变异表面糖蛋白为研究含磷脂酰肌醇的膜锚的结构和生物合成特征提供了一个模型系统。已经综述了磷脂酰肌醇锚定蛋白的发生和一般生物化学（Low et ul.，1986年; Cross，1987年;低。1987; Ferguson 61威廉姆斯，1988）。介绍了T.通过多种化学修饰和成分分析建立了布鲁氏菌变异体表面糖蛋白（VSG）膜锚（保持器，1983，198.5; Ferguson 61 Cross，1984; Ferguson等，198 Su，h），其表明所述锚是甘露糖-。含有半乳糖和葡糖胺的糖基磷脂酰肌醇（GPI）部分，通过乙醇胺桥连接到蛋白质a-COOH基团。VSG变体MlTat的GPI锚的完整结构。L. 4最近已经通过二维1H NMR、气相色谱-质谱、化学修饰和外切糖苷酶消化的组合来确定（Ferguson等人，1988）（见图1）锚显示
The cell surface integral membrane glycoproteins of eukaryotes can be classified with respect to the mode of attachment to the plasma membrane. The major class employs one or more transmembrane hydrophobic peptide sequences; the minor class is anchored via a covalent linkage to a phosphatidylinositol phospholipid. The latter anchor appears to be particularly common in protozoa. The variant surface glycoproteins of Trypunosomu brucei present a model system for the study of the structural and biosynthetic features of phosphatidylinositol-containing membrane anchors. The occurrence and general biochemistry of phosphatidylinositol-anchored proteins has been reviewed (Low et ul., 1986; Cross, 1987; Low. 1987; Ferguson 61 Williams, 1988). The basic arrangement of the T. brucei variant surface glycoprotein (VSG) membrane anchor was established by a variety of chemical modifications and by compositional analysis (Holder, 1983, 198.5; Ferguson 61 Cross, 1984; Ferguson et ul., 198Su, h) which showed that the anchor was a mannose-. galactose-and glucosamine-containing glycosylphosphatidylinositol (GPI) moiety, linked to the protein a-COOH group via an ethanolamine bridge. The complete structure of the GPI anchor of VSG variant MlTat. l. 4 has recently been determined by a combination of two-dimensional ‘H nmr, gas chromatography-mass spectrometry, chemical modifications and exoglycosidase digestion (Ferguson et ul., 1988)(see Fig. 1) The anchor displays