Separating the inseparable: the metabolomic analysis of plant-pathogen interactions.
Separating the inseparable: the metabolomic analysis of plant-pathogen interactions.
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DOI：
10.1007/978-1-61779-594-7_3
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Mur, Luis A J
中科院分区：
文献类型：
--
作者：
Allwood, J William;Heald, Jim;Mur, Luis A J
植物与微生物的相互作用，无论是致病的还是共生的，都对植物的生理和生产力产生重大影响。这种相互作用的分析代表了一个特殊的挑战，代谢组学的方法，由于相互作用的合作伙伴之间的密切联系，加上代谢物的一般共性。我们在这里描述了一种方法的基础上共培养的拟南芥细胞培养物和细菌植物病原体，以评估双方相互作用的合作伙伴，我们称之为双代谢组学的代谢组。
Plant-microbe interactions-whether pathogenic or symbiotic-exert major influences on plant physiology and productivity. Analysis of such interactions represents a particular challenge to metabolomic approaches due to the intimate association between the interacting partners coupled with a general commonality of metabolites. We here describe an approach based on co-cultivation of Arabidopsis cell cultures and bacterial plant pathogens to assess the metabolomes of both interacting partners, which we refer to as dual metabolomics.