Collection and extraction of saliva DNA for next generation sequencing.
Collection and extraction of saliva DNA for next generation sequencing.
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DOI：
10.3791/51697
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发表时间：
2014-08-27
期刊：
影响因子：
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通讯作者：
Bartlett CW
中科院分区：
文献类型：
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作者：
Goode MR;Cheong SY;Li N;Ray WC;Bartlett CW
从唾液中提取DNA可以提供容易获得的高分子量DNA来源，几乎没有降解/片段化。该方案为唾液收集/储存和DNA提取提供了优化的参数，以具有足够的质量和数量，用于具有高质量要求的下游DNA测定。长摘要：在人类遗传学研究中，DNA的首选来源是血液或来自血液的细胞系，因为这些来源产生大量高质量的DNA。然而，从唾液中提取DNA可以产生高质量的DNA，几乎没有降解/片段化，这适用于各种DNA测定，而无需抽血师的费用，甚至可以通过邮件获得。然而，目前，文献中还没有提出用于下一代测序的唾液DNA收集/提取方案。该方案优化了唾液收集/储存和DNA提取的参数，使其具有足够的质量和数量，用于具有最高标准的DNA测定，包括微阵列基因分型和下一代测序。
DNA extraction from saliva can provide a readily available source of high molecular weight DNA, with little to no degradation/fragmentation. This protocol provides optimized parameters for saliva collection/storage and DNA extraction to be of sufficient quality and quantity for downstream DNA assays with high quality requirements. Long Abstract: The preferred source of DNA in human genetics research is blood, or cell lines derived from blood, as these sources yield large quantities of high quality DNA. However, DNA extraction from saliva can yield high quality DNA with little to no degradation/fragmentation that is suitable for a variety of DNA assays without the expense of a phlebotomist and can even be acquired through the mail. However, at present, no saliva DNA collection/extraction protocols for next generation sequencing have been presented in the literature. This protocol optimizes parameters of saliva collection/storage and DNA extraction to be of sufficient quality and quantity for DNA assays with the highest standards, including microarray genotyping and next generation sequencing.