Characterization of the microbial community of lotic organic aggregates ('river snow') in the Elbe River of Germany by cultivation and molecular methods
Characterization of the microbial community of lotic organic aggregates ('river snow') in the Elbe River of Germany by cultivation and molecular methods
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DOI：
10.1016/s0168-6496(00)00056-8
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发表时间：
2000-08-01
影响因子：
4.2
通讯作者：
Szewzyk, U
中科院分区：
文献类型：
--
作者：
Böckelmann, U;Manz, W;Szewzyk, U
使用需氧和厌氧栽培技术、基于 16S rDNA 的系统发育和荧光原位杂交来描述从易北河获得的大量微生物聚集体（“河雪”）的系统发育多样性和生理多功能性。在这一年里，“河雪”社区发生了变化。其特点是春季细菌多样性丰富，夏季藻类占优势，秋冬季细菌细胞总数减少。在所有“河雪”样本中，超过 70% 使用通用 DNA 染色剂 DAPI 计数的细菌也与细菌特异性探针 EUB338 杂交。使用一整套特定 rRNA 靶向探针对细菌“河雪群落”进行原位分析，揭示了种群动态受季节因素的影响。在所有季节中，β-变形菌门都是数量上最重要的细菌群，占细胞总数的 54%。与此相反，其他主要细菌谱系的相对丰度范围从浮霉菌目的 2% 到噬细胞黄杆菌的 36%。在需氧和厌氧条件下使用多种不同培养基培养“河雪”，分离出 40 种新菌株。表型和系统发育分析表明，这些新菌株大多是属于不同细菌门的未知生物。应用新开发的特异性寡核苷酸探针对培养的细菌进行了验证。包括梭菌和数量丰富的β-变形菌，作为“河雪”群落的相关原位成员。 (C) 2000 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
Aerobic and anaerobic cultivation techniques, 16S rDNA-based phylogeny, and fluorescent in situ hybridization were used to describe the phylogenetic diversity and physiological versatility of lotic microbial aggregates ('river snow') obtained from the river Elbe. In the course of the year the 'river snow' community changed. It was characterized by a great bacterial diversity in spring, the predominant occurrence of algae in summer and reduction of the total bacterial cell count in autumn and winter. In all 'river snow' samples, more than 70% of the bacteria counted with the general DNA stain DAPI also hybridized with the Bacteria-specific probe EUB338. In situ analysis of the bacterial 'river snow community with a comprehensive suite of specific rRNA-targeted probes revealed population dynamics to be governed by seasonal factors. During all seasons, beta-Proteobacteria constituted the numerically most important bacterial group forming up to 54% of the total cell counts. In contrast to this, the relative abundance of other major bacterial lineages ranged from 2% for the order Planctomycetales to 36% for Cytophaga-Flavobacteria. Cultivation of 'river snow' under aerobic and anaerobic conditions with a variety of different media resulted in the isolation of 40 new bacterial strains. Phenotypic and phylogenetic analyses revealed these new strains to be mostly unknown organisms affiliated to different bacterial phyla. Application of newly developed specific oligonucleotide probes proved the cultivated bacteria. including clostridia and the numerically abundant beta-Proteobacteria, as relevant in situ members of the;river snow' community. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.