MUC4 and MUC5B transcripts are the prevalent mucin messenger ribonucleic acids of the human endocervix
MUC4 and MUC5B transcripts are the prevalent mucin messenger ribonucleic acids of the human endocervix
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DOI：
10.1095/biolreprod60.1.58
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发表时间：
1999-01-01
影响因子：
3.6
通讯作者：
Hill, JA
中科院分区：
文献类型：
--
作者：
Gipson, IK;Spurr-Michaud, S;Hill, JA
宫颈上皮分泌的粘蛋白保护生殖道上皮表面免受病原体侵袭，并调节精子进入子宫。三种大的凝胶形成粘蛋白，MUC 5AC、5 B和6，由宫颈上皮表达，MUC 4也是如此，MUC 4是一种相对未表征的粘蛋白，仅报道了其串联重复序列。我们试图确定这些粘蛋白基因转录本的相对丰度，并将其表达与血液孕酮和雌二醇。在月经周期的连续阶段从六名受试者中获得样品。在半定量逆转录-聚合酶链反应中使用MUC 4、5AC、5 B和6的非串联重复序列的引物，以确定与β 2-微球蛋白信息控制相关的每个粘蛋白基因的相对丰度。为了设计来自MUC 4的非串联重复区的引物，从而可以定量MUC 4的信息水平，我们获得了MUC 4基因组克隆的串联重复序列5 ′的大约2.7-组氨酸酶非串联重复序列。该序列显示缺乏富含半胱氨酸的D-结构域，富含丝氨酸和苏氨酸。半定量聚合酶链反应分析表明，人宫颈管的主要粘蛋白转录MUC 4和MUC 5 B，MUC 4占主导地位的21个样本中的15。当与血浆类固醇水平相关时，MUC 4和MUC 5 B的信使水平与孕酮水平呈负相关。
Mucins secreted by the endocervical epithelium protect the surfaces of the reproductive tract epithelium from pathogen penetrance and modulate sperm entry into the uterus. Three large gel-forming mucins, MUCs 5AC, 5B, and 6, are expressed by the endocervical epithelium, as is MUC4, a relatively uncharacterized mucin for which only tandem repeat sequence has been reported. We sought to determine the relative abundance of each of these mucin gene transcripts and to relate their expression to blood progesterone and estradiol. Samples were obtained from six subjects at successive stages in the menstrual cycle. Primers to nontandem repeat sequences of MUCs 4, 5AC, 5B, and 6 were used in semiquantitative reverse transcription-polymerase chain reaction to determine relative abundance of each mucin gene in relation to beta(2)-microglobulin message control. in order to design primers from a nontandem repeat region of MUC4 so that MUC4 message levels could be quantitated, we obtained approximately 2.7-kilobase nontandem repeat sequence 5' to the tandem repeat sequence of a MUC4 genomic clone. The sequence showed lack of cysteine-rich D-domains and was rich in serine and threonine. Semiquantitative polymerase chain reaction analyses indicated that the principal mucin transcripts of human endocervix are MUC4 and MUC5B, with MUC4 predominant in 15 of 21 samples. When correlated with plasma steroid levels, message levels of both MUC4 and MUC5B were inversely related to progesterone levels.