Neuronal Density Determines Network Connectivity and Spontaneous Activity in Cultured Hippocampus
Neuronal Density Determines Network Connectivity and Spontaneous Activity in Cultured Hippocampus
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DOI：
10.1152/jn.00914.2009
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发表时间：
2010-08-01
影响因子：
2.5
通讯作者：
Segal, Menahem
中科院分区：
文献类型：
--
作者：
Ivenshitz, Miriam;Segal, Menahem
Ivenshitz M，Segal M。神经元密度决定培养海马中的网络连接性和自发活动。 J Neurophysiol 104: 1052-1060, 2010。首次发表于 2010 年 6 月 16 日； doi：10.1152/jn.00914.2009。在培养的分离海马神经元中研究了神经元密度对进化网络的形态和功能属性的影响。不同密度的电镀会影响神经元之间的连接性，使得稀疏网络在记录的神经元对之间表现出更强的突触连接。这与稀疏培养物中突发大小增加但突发频率降低的自发网络活动的不同模式相关。神经元密度还影响这些神经元的树突和棘的形态，使得稀疏神经元具有更简单的树突树和更少的树突棘。此外，对转染 PSD95 的神经元的分析表明，在稀疏培养物中，突触在树突轴上形成，而在密集培养物中，突触主要在棘头上形成。这些实验提供了关于神经元密度在群体活动中的作用的重要线索，并且应该对控制神经元网络连接的规则产生新的见解。
Ivenshitz M, Segal M. Neuronal density determines network connectivity and spontaneous activity in cultured hippocampus. J Neurophysiol 104: 1052-1060, 2010. First published June 16, 2010; doi: 10.1152/jn.00914.2009. The effects of neuronal density on morphological and functional attributes of the evolving networks were studied in cultured dissociated hippocampal neurons. Plating at different densities affected connectivity among the neurons, such that sparse networks exhibited stronger synaptic connections between pairs of recorded neurons. This was associated with different patterns of spontaneous network activity with enhanced burst size but reduced burst frequency in the sparse cultures. Neuronal density also affected the morphology of the dendrites and spines of these neurons, such that sparse neurons had a simpler dendritic tree and fewer dendritic spines. Additionally, analysis of neurons transfected with PSD95 revealed that in sparse cultures the synapses are formed on the dendritic shaft, whereas in dense cultures the synapses are formed primarily on spine heads. These experiments provide important clues on the role of neuronal density in population activity and should yield new insights into the rules governing neuronal network connectivity.