Fine mapping of the friend retrovirus resistance gene, Rfv3, on mouse chromosome 15
Fine mapping of the friend retrovirus resistance gene, Rfv3, on mouse chromosome 15
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DOI：
10.1128/jvi.73.9.7848-7852.1999
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发表时间：
1999-09-01
影响因子：
5.4
通讯作者：
Chesebro, B
中科院分区：
文献类型：
--
作者：
Super, HJ;Hasenkrug, KJ;Chesebro, B
Rfv3是一种宿主抗性基因，通过未知机制控制Friend逆转录病毒感染后恢复所需的病毒中和抗体反应的发展。Rfv3基因先前被定位于15号染色体的约20厘摩（cM）区域。由于缺少微卫星标记和Rfv3表型中的泄漏，无法进行更精确的定位，这阻碍了单个重组小鼠的确定性表型。在本研究中，我们克服了这些困难，利用7个新的微卫星标记的Rfv3区域和后代测试，以准确地确定重组小鼠的Rfv3表型。相关交叉的详细连锁分析将Rfv3的位置缩小到0.83 cM区域。紧密连锁的基因在种间回交面板的映射允许我们排除两个以前的候选基因，Ly6和Wnt7b。这些研究还首次表明，Hsf1基因映射到Rfv3连锁的基因簇，包括Il2rb，Il3rb和Pdgfb。Rfv3定位于小于1cM的区域，这使得尝试通过物理方法克隆Rfv3成为可能。
Rfv3 is a host resistance gene that operates through an unknown mechanism to control the development of the virus-neutralizing antibody response required for recovery from infection with Friend retrovirus. The Rfv3 gene was previously mapped to an approximately 20-centimorgan (cM) region of chromosome 15. More refined mapping was not possible, due to a lack of microsatellite markers and leakiness in the Rfv3 phenotype, which prevented definitive phenotyping of individual recombinant mice. In the present study, we overcame these difficulties by taking advantage of seven new microsatellite markers in the Rfv3 region and by using progeny tests to accurately determine the Rfv3 phenotype of recombinant mice. Detailed linkage analysis of relevant crossovers narrowed the location of Rfv3 to a 0.83-cM region. Mapping of closely linked genes in an interspecific backcross panel allowed us to exclude two previous candidate genes, Ly6 and Wnt7b. These studies also showed for the first time that the Hsf1 gene maps to the Rfv3-linked cluster of genes including Il2rb, Il3rb, and Pdgfb. This localization of Rfv3 to a region of less than 1 cM now makes it feasible to attempt the cloning of Rfv3 by physical methods.