A trial of production of the plant-derived high-value protein in a plant factory Photosynthetic photon fluxes affect the accumulation of recombinant miraculin in transgenic tomato fruits
A trial of production of the plant-derived high-value protein in a plant factory Photosynthetic photon fluxes affect the accumulation of recombinant miraculin in transgenic tomato fruits
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DOI：
10.4161/psb.6.8.16373
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发表时间：
2011-01-01
影响因子：
2.9
通讯作者：
Ezura, Hiroshi
中科院分区：
文献类型：
--
作者：
Kato, Kazuhisa;Maruyama, Shinichi;Ezura, Hiroshi
植物科学的最终目标之一是检验基础科学得出的假设，并将其应用于农业和工业。植物工厂是本试验的理想系统之一。环境因素既影响植物产量，也影响转基因植物中用于工业应用的重组蛋白的积累。然而，在被称为植物工厂的封闭栽培系统中研究重组蛋白的植物生产力的报道很少。为了研究光合光子通量（PPF）对转基因番茄果实产量和重组味修饰糖蛋白miraculin积累的影响，在植物工厂中以100 ~ 400 (μ mol m（-2)s(-1)）不同的PPF培养植株。单位能量消耗的奇迹蛋白产量在PPF100时最高，而单位面积的奇迹蛋白产量在PPF300时最高。转基因番茄果实中重组奇迹蛋白的商业化生产在很大程度上取决于植物工厂的光照条件。我们的试验将有助于考虑工厂生产高价值材料的利润与电力成本之间的权衡。
One of the ultimate goals of plant science is to test a hypothesis obtained by basic science and to apply it to agriculture and industry. A plant factory is one of the ideal systems for this trial. Environmental factors affect both plant yield and the accumulation of recombinant proteins for industrial applications within transgenic plants. However, there have been few reports studying plant productivity for recombinant protein in closed cultivation systems called plant factories.To investigate the effects of photosynthetic photon flux (PPF) on tomato fruit yield and the accumulation of recombinant miraculin, a taste-modifying glycoprotein, in transgenic tomato fruits, plants were cultivated at various PPFs from 100 to 400 (mu mol m(-2)s(-1)) in a plant factory. Miraculin production per unit of energy used was highest at PPF100, although miraculin production per unit area was highest at PPF300. The commercial productivity of recombinant miraculin in transgenic tomato fruits largely depended on light conditions in the plant factory. Our trial will be useful to consider the trade-offs between the profits from production of high-value materials in plants and the costs of electricity.