A specific, nonproliferative role for E2F-5 in choroid plexus function revealed by gene targeting
A specific, nonproliferative role for E2F-5 in choroid plexus function revealed by gene targeting
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DOI：
10.1101/gad.12.8.1092
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发表时间：
1998-04-15
影响因子：
10.5
通讯作者：
Livingston, DM
中科院分区：
文献类型：
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作者：
Lindeman, GJ;Dagnino, L;Livingston, DM
已经产生了纯合E2 F-5敲除胚胎和小鼠。虽然胚胎发育似乎正常，但新生小鼠发生了非梗阻性脑积水，表明脑脊液（CSF）产生过多。虽然CSF产生脉络丛显示正常的细胞组织，它含有丰富的电子透明上皮细胞，与过量的CSF分泌活动一致。此外，E2 F-5 CNS在正常动物中的表达主要局限于脉络丛，在纯合敲除胚胎成纤维细胞中细胞周期动力学不受干扰。因此，E2 F-5不是细胞增殖所必需的，而是影响分化的神经组织的分泌行为。
Homozygous E2F-5 knockout embryos and mice have been generated. Although embryonic development appeared normal, newborn mice developed nonobstructive hydrocephalus, suggesting excessive cerebrospinal fluid (CSF) production. Although the CSF-producing choroid plexus displayed normal cellular organization, it contained abundant electron-lucent epithelial cells, consistent with excessive CSF secretory activity. Moreover, E2F-5 CNS expression in normal animals was largely confined to the choroid plexus, Cell cycle kinetics were not perturbed in homozygous knockout embryo fibroblasts. Thus, E2F-5 is not essential for cell proliferation, Rather, it affects the secretory behavior of a differentiated neural tissue.